Journal Article

Enhanced production of iturin A by strengthening fatty acid synthesis modules in Bacillus amyloliquefaciens

Cai, Dongbo, Wang, Dong, Shi, Jiao, Yang, Zhifan, She, Menglin, Xiong, Min, Chen, Shouwen, Shen, Guoming, Gao, Lin, Gao, Jiaming, Zhang, Min

2022

Overview

Iturin A is a biosurfactant with various applications, and its low synthesis capability limits its production and application development. Fatty acids play a critical role in cellular metabolism and target product syntheses, and the relationship between fatty acid supplies and iturin A synthesis is unclear. In this study, we attempted to increase iturin A production via strengthening fatty acid synthesis pathways in Bacillus amyloliquefaciens . First, acetyl-CoA carboxylase AccAD and ACP S-malonyltransferase fabD were overexpressed via promoter replacement, and iturin A yield was increased to 1.36 g/L by 2.78-fold in the resultant strain HZ-ADF1. Then, soluble acyl-ACP thioesterase derived from Escherichia coli showed the best performance for iturin A synthesis, as compared to those derived from B. amyloliquefaciens and Corynebacterium glutamicum , the introduction of which in HZ-ADF1 further led to a 57.35% increase of iturin A yield, reaching 2.14 g/L. Finally, long-chain fatty acid-CoA ligase LcfA was overexpressed in HZ-ADFT to attain the final strain HZ-ADFTL2, and iturin A yield reached 2.96 g/L, increasing by 6.59-fold, and the contents of fatty acids were enhanced significantly in HZ-ADFTL2, as compared to the original strain HZ-12. Taken together, our results implied that strengthening fatty acid supplies was an efficient approach for iturin A production, and this research provided a promising strain for industrial production of iturin A.

• Publisher: Frontiers Media SA,Frontiers Media S.A
• Subject: Acetyl-CoA carboxylase / Bacillus amyloliquefaciens / Bioengineering and Biotechnology / E coli / fatty acid / Fatty acids / Fermentation / gene expression / Genes / Genetic engineering / Genomes / Iturin A / metabolic engineering / Metabolism / Metabolites / Plasmids