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Optimized dCas9 programmable transcriptional activators for plants
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Journal Article
Optimized dCas9 programmable transcriptional activators for plants
2024
Overview
Understanding how gene expression impacts plant development and physiology is important for crop engineering. Programmable transcriptional activators (PTAs), including CRISPR-Cas activators, have relied on a limited number of transcriptional activation domains (ADs) (Casas-Mollano et al., 2020; Lowder et al., 2018; Pan et al., 2021; Papikian et al., 2019). Usually, the VP64 domain, derived from herpes simplex virus, is fused to a DNA-binding domain to activate target gene expression. In dCas9-based PTAs, binding to a target DNA sequence is afforded by the dCas9-sgRNA ribonucleoprotein. We reasoned there was considerable space for PTA improvement by replacing VP64 with a plant-derived AD.
Publisher
John Wiley & Sons, Inc,Wiley,John Wiley and Sons Inc
