Journal Article

Somatotopic localization of c-Fos expression in the spinal cord in response to noxious heat sensation

Khan, Gulfaraz, Javed, Hayate, Shehab, Safa

2022

Overview

Results showed that 78% of shEGFP +ve cells were positive for excitatory neurons which contain Vglut2 (vesicular glutamate transporter) whereas 22% were positive for inhibitory neurons which contain GAD65/67 (glutamate decarboxylase 65/67) (Figures 1G,H, in Wang et al., 2022). [...]following noxious heat, 33% of c-Fos +ve neurons also expressed ErbB4 (Figures 1M,N, in Wang et al., 2022). The medial part of the dorsal horn receives the termination of the primary afferents from the limbs through the ventral ramus, while the lateral part receives the termination of the primary afferents from the skin of the back through the dorsal ramus (Figure 1A). [...]exposing the hind paw of mice to noxious heat stimuli, as carried out by Wang et al. Scale bar = 250 [mu]m. Localization of c-Fos expression in response to noxious heat stimulation of the hind paw We used two methods to localize the distribution of c-Fos expression in response to noxious heat stimulation in adult male mice (C57BL/6): (1) Intraplantar injection of resiniferatoxin (45 μl of 0.001%), as an agonist of heat receptor TRPV1 (transient receptor potential vanilloid type 1, Caterina et al., 2000) into the left hind paw. All experimental procedures were approved by the Animal Ethics Committee of the CMHS, UAE University, and were performed in accordance with the guidelines of the European Communities Council Directive of November 24, 1986 (86/609/EEC).

• Publisher: Frontiers Research Foundation
• Subject: c-Fos protein / Capsaicin receptors / Conflicts of interest / Dorsal horn / ErbB-2 protein / Glutamate decarboxylase / Glutamic acid transporter / Grants / Heat / Heat stimuli / Localization / Neurons / Resiniferatoxin / Sensation / Skin / Spinal cord / Transient receptor potential proteins