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Enhanced base editing by co-expression of free uracil DNA glycosylase inhibitor
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Journal Article
Enhanced base editing by co-expression of free uracil DNA glycosylase inhibitor
2017
Overview
Dear Editor, Base editors （BEs） have been recently developed by combining the APOBEC （apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like）/AID （acti- vation-induced deaminase） cytidine deaminase family members [1] with the CRISPR./Cas9 system to perform targeted C-to-T base editing [2-8]. Mechanistically, Cas9 variant-fused APOBEC/AID is directed to target site by sgRNA, introducing C-to-T substitution at the single-base level [2-4]. Compared to earlier generations of BEs （BE1 and BE2）, the latest BE3 achieved much higher base editing frequencies by substituting catalyti- cally-dead Cas9 （dCas9） with Cas9 nickase （nCas9） [2].
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
