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POLθ-mediated end joining is restricted by RAD52 and BRCA2 until the onset of mitosis
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POLθ-mediated end joining is restricted by RAD52 and BRCA2 until the onset of mitosis
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Journal Article
POLθ-mediated end joining is restricted by RAD52 and BRCA2 until the onset of mitosis
2021
Overview
BRCA2-mutant cells are defective in homologous recombination, making them vulnerable to the inactivation of other pathways for the repair of DNA double-strand breaks (DSBs). This concept can be clinically exploited but is currently limited due to insufficient knowledge about how DSBs are repaired in the absence of BRCA2. We show that DNA polymerase θ (POLθ)-mediated end joining (TMEJ) repairs DSBs arising during the S phase in BRCA2-deficient cells only after the onset of the ensuing mitosis. This process is regulated by RAD52, whose loss causes the premature usage of TMEJ and the formation of chromosomal fusions. Purified RAD52 and BRCA2 proteins both block the DNA polymerase function of POLθ, suggesting a mechanism explaining their synthetic lethal relationships. We propose that the delay of TMEJ until mitosis ensures the conversion of originally one-ended DSBs into two-ended DSBs. Mitotic chromatin condensation might further serve to juxtapose correct break ends and limit chromosomal fusions.
Löbrich and colleagues report that RAD52 and BRCA2 limit polymerase θ activity until the onset of mitosis to ensure double-strand breaks arising in the S phase are repaired by polymerase θ-mediated end joining in mitosis, not in S phase.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ 13/89
/ 14/63
/ 38/77
/ 82/29
/ 82/83
/ Biomedical and Life Sciences
/ DNA
/ DNA-Directed DNA Polymerase - genetics
/ DNA-Directed DNA Polymerase - metabolism
/ Homology
/ Humans
/ Mitosis
/ Rad52 DNA Repair and Recombination Protein - genetics
/ Rad52 DNA Repair and Recombination Protein - metabolism
/ S phase
