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Molecular diagnosis of Entamoeba spp. versus microscopy in the Great Cairo
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Molecular diagnosis of Entamoeba spp. versus microscopy in the Great Cairo
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Journal Article
Molecular diagnosis of Entamoeba spp. versus microscopy in the Great Cairo
2017
Overview
Amoebiasis is a human disease produced by
Entamoeba histolytica
which causes widespread mortality and morbidity worldwide through diarrheal disease and abscess establishment in parenchymal tissues such as liver, lung, and brain. The true prevalence of infection is unknown for most areas of the world due to the difficulty to characterise
Entamoeba histolytica
versus other non-pathogenic amoebas with identical morphology, as E
ntamoeba dispar
, and E
ntamoeba moshkovskii
. To overcome microscopy misidentification issues, we tested a nested multiplex polymerase chain reaction (PCR) and a real-time PCR on 194 stool samples collected from incoming dysentery patients in Cairo hospitals diagnosed with
E. histolytica
by microscopy. Nested PCR showed only 20 (10.3%) samples positive to
E. histolytica
and 17 (8.7%) to
E. dispar
. The real-time PCR detected only 19 and 11 samples positive to
E. histolytica
and
E. dispar
respectively, showing less sensitivity than the nested PCR. The data show that prevalence of
E. histolytica
in Cairo is lower when specific diagnosis methods are used instead of traditional microscopy, allowing to differentiate between morphologically identical human amoebas species.
Publisher
Springer International Publishing,De Gruyter,Springer Nature B.V
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