Journal Article

De Novo Genome and Transcriptome Assembly of the Canadian Beaver (Castor canadensis)

Wang, Zhuozhi, Apresto, Beverly, Dutton, Christopher J, Sung, Wilson W L, Coole, Matthew, Trost, Brett, Kerr, Kevin C R, Walker, Susan, Lim, Burton K, Shipstone, Arun, Ng, Karen, Yan Tong, Amy Hin, Hu, Zhizhou, Pullenayegum, Sanjeev, Tsoi, Fiona, Dawson, Travis J, Yuen, Ryan K C, Sundaravadanam, Yogi, Franke, Maria, Singh, Naina, Wang, Ting, Simpson, Jared T, Scherer, Stephen W, Lok, Si, Ryan, Carol Ann, Kaur, Gaganjot, Rapley, William, Chen, Nan, Paton, Tara A, Rostami, Pirooz, Buchanan, Janet A, Samler, Kozue, Ho, Karen, Engstrom, Mark D, Whitney, Joseph, Wintle, Richard F, Pereira, Sergio L

2017

Overview

The Canadian beaver (Castor canadensis) is the largest indigenous rodent in North America. We report a draft annotated assembly of the beaver genome, the first for a large rodent and the first mammalian genome assembled directly from uncorrected and moderate coverage (< 30 ×) long reads generated by single-molecule sequencing. The genome size is 2.7 Gb estimated by k-mer analysis. We assembled the beaver genome using the new Canu assembler optimized for noisy reads. The resulting assembly was refined using Pilon supported by short reads (80 ×) and checked for accuracy by congruency against an independent short read assembly. We scaffolded the assembly using the exon–gene models derived from 9805 full-length open reading frames (FL-ORFs) constructed from the beaver leukocyte and muscle transcriptomes. The final assembly comprised 22,515 contigs with an N50 of 278,680 bp and an N50-scaffold of 317,558 bp. Maximum contig and scaffold lengths were 3.3 and 4.2 Mb, respectively, with a combined scaffold length representing 92% of the estimated genome size. The completeness and accuracy of the scaffold assembly was demonstrated by the precise exon placement for 91.1% of the 9805 assembled FL-ORFs and 83.1% of the BUSCO (Benchmarking Universal Single-Copy Orthologs) gene set used to assess the quality of genome assemblies. Well-represented were genes involved in dentition and enamel deposition, defining characteristics of rodents with which the beaver is well-endowed. The study provides insights for genome assembly and an important genomics resource for Castoridae and rodent evolutionary biology.

• Publisher: Oxford University Press
• Subject: Beavers / Genomes