Journal Article

An organotypic atlas of human vascular cells

Cujba, Ana-Maria, Pritchard, Sophie, Toscano-Rivalta, Rebecca, Joseph, Philomeena D., Yang, Lu, Meyer, Kerstin B., Mach, Lukas, Petryszak, Robert, Kanemaru, Kazumasa, Kim, Chang N., Barnett, Sam N., Lee, Michael, Pett, J. Patrick, Miranda, Antonio M. A., Teichmann, Sarah A., Chowdhury, Rasheda A., Bolt, Liam, Cranley, James, Tudor, Catherine, Treutlein, Barbara, Gomes, Tomás, Li, Shuang, Perera, Shani, Campos, Lia, Brazovskaja, Agnieska, Tuong, Zewen K., Noseda, Michela, Kedlian, Veronika R., Randi, Anna M., Madissoon, Elo, Maceiras, Ana Raquel, Xu, Chuan, Polanski, Krzysztof, Elmentaite, Rasa, Oliver, Amanda J., Sarropoulos, Ioannis, Nowakowski, Tomasz J., Cakir, Batuhan, Huseynov, Alik, Prete, Martin

2024

Overview

The human vascular system, comprising endothelial cells (ECs) and mural cells, covers a vast surface area in the body, providing a critical interface between blood and tissue environments. Functional differences exist across specific vascular beds, but their molecular determinants across tissues remain largely unknown. In this study, we integrated single-cell transcriptomics data from 19 human organs and tissues and defined 42 vascular cell states from approximately 67,000 cells (62 donors), including angiotypic transitional signatures along the arterial endothelial axis from large to small caliber vessels. We also characterized organotypic populations, including splenic littoral and blood–brain barrier ECs, thus clarifying the molecular profiles of these important cell states. Interrogating endothelial–mural cell molecular crosstalk revealed angiotypic and organotypic communication pathways related to Notch, Wnt, retinoic acid, prostaglandin and cell adhesion signaling. Transcription factor network analysis revealed differential regulation of downstream target genes in tissue-specific modules, such as those of FOXF1 across multiple lung vascular subpopulations. Additionally, we make mechanistic inferences of vascular drug targets within different vascular beds. This open-access resource enhances our understanding of angiodiversity and organotypic molecular signatures in human vascular cells, and has therapeutic implications for vascular diseases across tissues. A vascular cell atlas integrating single-cell data of 19 organs and tissues from 62 donors identifies angiotypic and organotypic characteristics of endothelial and mural cells.

• Publisher: Nature Publishing Group US,Nature Publishing Group
• Subject: 631/114 / 631/337/2019 / 631/80 / Angiogenesis / Biomedical and Life Sciences / Biomedicine / Blood vessels / Blood Vessels - cytology / Blood-brain barrier / Blood-Brain Barrier - cytology / Blood-Brain Barrier - metabolism / Cancer Research / Cell adhesion / Cell interactions / Crosstalk / Endothelial cells / Endothelial Cells - metabolism / Gene regulation / Gene Regulatory Networks / Homeostasis / Humans / Infectious Diseases / Littoral environments / Lymphatic system / Metabolic Diseases / Molecular Medicine / Musculoskeletal system / Network analysis / Neurosciences / Organ Specificity - genetics / Organs / Retinoic acid / Signal Transduction / Signatures / Single-Cell Analysis / Subpopulations / Therapeutic targets / Tissues / Transcriptome / Transcriptomics / Vascular diseases / Vascular system / Veins & arteries / Wnt protein