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CHD3 chromatin-remodeling factor PICKLE regulates floral transition partially via modulating LEAFY expression at the chromatin level in Arabidopsis
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CHD3 chromatin-remodeling factor PICKLE regulates floral transition partially via modulating LEAFY expression at the chromatin level in Arabidopsis
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CHD3 chromatin-remodeling factor PICKLE regulates floral transition partially via modulating LEAFY expression at the chromatin level in Arabidopsis
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Journal Article
CHD3 chromatin-remodeling factor PICKLE regulates floral transition partially via modulating LEAFY expression at the chromatin level in Arabidopsis
2016
Overview
PICKLE （PKL）, a putative CHD3 chromatin remodeling factor, has been suggested to be involved in multiple processes in Ar- abidopsis. Here, we confirmed the late-flowering phenotype caused by pkl mutation with pkl mutants in two different ecotypes, and investigated the possible mechanisms that account for PKL regulation of flowering time. Quantitative RT-PCR and RNA-seq assays showed that expression of the LEAFY gene （LFY） and a number of LFY-regulated floral homeotic genes were down-regulated in seedlings of the pkl mutants. As predicted, overexpression of LFY restored normal flowering time of pkl mutants. Our results suggest that PKL may be involved in regulating flowering time via LFY expression. To uncover the un- derlying mechanism, ChIP-PCR using anti-PKL was performed on materials from three developmental stages of seedlings. Our results showed that PKL associated with the genomic sequences of LFY, particularly at 10-day and 25-day after germina- tion. We also showed that loss of PKL affected H3K27me3 level at the promoter of LFY. Taken together, our data suggest that transcriptional regulation of LFY at the chromatin level by PKL may at least partially account for the late-flowering phenotype of pkl mutants.
Publisher
Science China Press,Springer Nature B.V
Subject
/ Arabidopsis Proteins - genetics
/ Arabidopsis Proteins - physiology
/ Biomedical and Life Sciences
/ Flowers - growth & development
/ Gene Expression Regulation, Plant - physiology
/ LFY基因
/ Mutation
/ Real-Time Polymerase Chain Reaction
/ Transcription Factors - genetics
/ 叶表
/ 因子
/ 定量RT-PCR
/ 拟南芥
/ 染色质重塑
/ 水
/ 泡菜
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