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Voltage-gated K＋ channels in adipogenic differentiation of bone marrow-derived human mesenchymal stem cells
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Voltage-gated K＋ channels in adipogenic differentiation of bone marrow-derived human mesenchymal stem cells
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Voltage-gated K＋ channels in adipogenic differentiation of bone marrow-derived human mesenchymal stem cells
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Journal Article
Voltage-gated K＋ channels in adipogenic differentiation of bone marrow-derived human mesenchymal stem cells
2013
Overview
Aim： To determine the presence of voltage-gated K＋ （Kv） channels in bone marrow-derived human mesenchymal stem cells （hMSCs） and their impact on differentiation of hMSCs into adipocytes. Methods： For adipogenic differentiation, hMSCs were cultured in adipogenic medium for 22 d. The degrees of adipogenic differentiation were examined using Western blot, Oil Red 0 staining and Alamar assay. The expression levels of Kv channel subunits Kvl.1, Kvl.2, Kvl.3, Kvl.4, Kv2.1, Kv3.1, Kv3.3, Kv4.2, Kv4.3, and Kv9.3 in the cells were detected using RT-PCR and Western blot analysis. Results： The expression levels of Kv2.1 and Kv3.3 subunits were markedly increased on d 16 and 22. in contrast, the expression levels of other Kv channel subunits, including Kvl.1, Kvl.2, Kvl.3, Kvl.4, Kv4.2, Kv4.3, and Kv9.3, were decreased as undifferentiated hMSCs differentiated into adipocytes. Addition of the Kv channel blocker tetraethylammonium （TEA, 10 mmol/L） into the adipogenic medium for 6 or 12 d caused a significant decrease, although not complete, in lipid droplet formation and adipocyte fatty acid-binding protein 2 （aP2） expressions. Addition of the selective Kv2.1 channel blocker guangxitoxin （GxTX-1, 40 nmol/L） into the adipogenic medium for 21 d also suppressed adipogenic differentiation of the cells. Conclusion： The results demonstrate that subsets of Kv channels including Kv2.1 and Kv3.3 may play an important role in the differentiation of hMSCs into adipocytes.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ Biomedical and Life Sciences
/ blot分析
/ Bone Marrow Cells - cytology
/ Gene Expression Regulation, Developmental
/ Humans
/ KVL
/ Mesenchymal Stromal Cells - cytology
/ Mesenchymal Stromal Cells - drug effects
/ Mesenchymal Stromal Cells - metabolism
/ Original
/ Potassium Channel Blockers - pharmacology
/ Potassium Channels, Voltage-Gated - antagonists & inhibitors
/ Potassium Channels, Voltage-Gated - genetics
/ Potassium Channels, Voltage-Gated - metabolism
/ RT-PCR
/ Tetraethylammonium - pharmacology
/ Vaccine
/ 电压门控
/ 脂肪细胞分化
/ 脂肪酸结合蛋白
/ 钾通道
/ 骨髓间充质干细胞
