Asset Details
Do you wish to reserve the book?
Bacterial cell surface characterization by phage display coupled to high-throughput sequencing
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Bacterial cell surface characterization by phage display coupled to high-throughput sequencing
Do you wish to request the book?
Bacterial cell surface characterization by phage display coupled to high-throughput sequencing
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Bacterial cell surface characterization by phage display coupled to high-throughput sequencing
2024
Overview
The remarkable capacity of bacteria to adapt in response to selective pressures drives antimicrobial resistance.
Pseudomonas aeruginosa
illustrates this point, establishing chronic infections during which it evolves to survive antimicrobials and evade host defenses. Many adaptive changes occur on the
P. aeruginosa
cell surface but methods to identify these are limited. Here we combine phage display with high-throughput DNA sequencing to create a high throughput, multiplexed technology for surveying bacterial cell surfaces, Phage-seq. By applying phage display panning to hundreds of bacterial genotypes and analyzing the dynamics of the phage display selection process, we capture important biological information about cell surfaces. This approach also yields camelid single-domain antibodies that recognize key
P. aeruginosa
virulence factors on live cells. These antibodies have numerous potential applications in diagnostics and therapeutics. We propose that Phage-seq establishes a powerful paradigm for studying the bacterial cell surface by identifying and profiling many surface features in parallel.
Methods to identify bacterial cell surface adaptations are limited. The authors combine phage display with high-throughput DNA sequencing to create a highly-multiplexed technology for surveying bacterial cell surfaces.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 45/23
/ 45/77
/ 82/1
/ 82/80
/ 82/83
/ 96
/ Antigens
/ Bacteria
/ Camelids
/ Cell Surface Display Techniques - methods
/ Cells
/ DNA
/ High-Throughput Nucleotide Sequencing - methods
/ Humanities and Social Sciences
/ Medicine
/ Panning
/ Phages
/ Proteins
/ Pseudomonas aeruginosa - genetics
/ Reagents
/ Science
We currently cannot retrieve any items related to this title. Kindly check back at a later time.