Journal Article

Spiraea prunifolia var. simpliciflora Attenuates Oxidative Stress and Inflammatory Responses in a Murine Model of Lipopolysaccharide-Induced Acute Lung Injury and TNF-α-Stimulated NCI-H292 Cells

Jeon, Da-Bin, Shin, Han-Gyo, Lee, Woo-Song, Ha, Ji-Hye, Kim, Ju-Hong, Kwon, Hyung-Jun, Ryu, Young-Bae, Jeong, Seong-Hun, Lee, In-Chul, Park, Ji-Young, Kim, Sung-Hwan, Jang, Hyun-Jae, Jung, Kyungsook, Kim, Hyeon-Young, Lee, Ba-Wool

2020

Overview

Spiraea prunifolia var. simpliciflora (SP) is traditionally used as an herbal remedy to treat fever, malaria, and emesis. This study aimed to evaluate the anti-oxidative and anti-inflammatory properties of the methanol extract of SP leaves in tumor necrosis factor (TNF)-α-stimulated NCI-H292 cells and in a lipopolysaccharide (LPS)-induced acute lung injury (ALI) mouse model. SP decreased the number of inflammatory cells and the levels of TNF-α, interleukin (IL)-1β, and IL-6 in the bronchoalveolar lavage fluid, and inflammatory cell infiltration in the lung tissues of SP-treated mice. In addition, SP significantly suppressed the mRNA and protein levels of TNF-α, IL-1β, and IL-6 in TNF-α-stimulated NCI-H292 cells. SP significantly suppressed the phosphorylation of the mitogen-activated protein kinases (MAPKs) and p65-nuclear factor-kappa B (NF-κB) in LPS-induced ALI mice and TNF-α-stimulated NCI-H292 cells. SP treatment enhanced the nuclear translocation of nuclear factor erythroid 2-related factor (Nrf2) with upregulated antioxidant enzymes and suppressed reactive oxygen species (ROS)-mediated oxidative stress in the lung tissues of LPS-induced ALI model and TNF-α-stimulated NCI-H292 cells. Collectively, SP effectively inhibited airway inflammation and ROS-mediated oxidative stress, which was closely related to its ability to induce activation of Nrf2 and inhibit the phosphorylation of MAPKs and NF-κB. These findings suggest that SP has therapeutic potential for the treatment of ALI.

• Publisher: MDPI,MDPI AG
• Subject: acute lung injury / animal models / anti-inflammatory activity / anti-oxidant activity / antioxidant enzymes / inflammation / interleukin-1beta / interleukin-6 / leaves / lipopolysaccharides / lungs / messenger RNA / methanol / mice / mitogen-activated protein kinase / nuclear factor erythroid 2-related factor / nuclear factor-kappab / oxidative stress / phosphorylation / plant extracts / protein content / reactive oxygen species / Spiraea / spiraea prunifolia var. simpliciflora / therapeutics / traditional medicine / transcription factor NF-kappa B / tumor necrosis factor-alpha