Journal Article

Transcriptome analysis of Pennisetum americanum × Pennisetum purpureum and Pennisetum americanum leaves in response to high-phosphorus stress

Zhao, Lili, Huang, Lei, Liu, Xiaoyan, Zhao, Xin, Wang, Puchang

2024

Overview

Excessive phosphorus (P) levels can disrupt nutrient balance in plants, adversely affecting growth. The molecular responses of Pennisetum species to high phosphorus stress remain poorly understood. This study examined two Pennisetum species, Pennisetum americanum × Pennisetum purpureum and Pennisetum americanum , under varying P concentrations (200, 600 and 1000 µmol·L − 1 KH 2 PO 4 ) to elucidate transcriptomic alterations under high-P conditions. Our findings revealed that P. americanum exhibited stronger adaption to high-P stress compared to P. americanum × P. purpureum . Both species showed an increase in plant height and leaf P content under elevated P levels, with P. americanum demonstrating greater height and higher P content than P. americanum × P. purpureum . Transcriptomic analysis identified significant up- and down-regulation of key genes (e.g. SAUR , GH3 , AHP , PIF4 , PYL , GST , GPX , GSR , CAT , SOD1 , CHS , ANR , P5CS and PsbO ) involved in plant hormone signal transduction, glutathione metabolism, peroxisomes, flavonoid biosynthesis, amino acid biosynthesis and photosynthesis pathways. Compared with P. americanum × P. purpureum , P. americanum has more key genes in the KEGG pathway, and some genes have higher expression levels. These results contribute valuable insights into the molecular mechanisms governing high-P stress in Pennisetum species and offer implications for broader plant stress research.

• Publisher: BioMed Central,Springer Nature B.V,BMC
• Subject: Agricultural production / Agriculture / Amino acids / Biomass energy / Biomedical and Life Sciences / Biosynthesis / Cenchrus americanus / Cenchrus purpureus / Efficiency / Fertilizers / Flavonoids / Gene expression / Gene Expression Profiling / Gene Expression Regulation, Plant / Gene regulation / Genes / Genes, Plant / Glutathione / High-phosphorus stress / Leaves / Life Sciences / Metabolism / Molecular modelling / Nutrient balance / Pennisetum / Pennisetum - genetics / Pennisetum - metabolism / Pennisetum americanum / Pennisetum americanum × Pennisetum purpureum / Pennisetum purpureum / peroxisomes / Phosphorus / Phosphorus - metabolism / Photosynthesis / Physiology / Plant growth / plant height / Plant hormones / Plant Leaves - genetics / Plant Leaves - metabolism / Plant Sciences / Plant stress / Plants (botany) / Potassium phosphate / Potassium phosphates / Seeds / Signal transduction / Software / species / Stress, Physiological - genetics / Superoxide dismutase / Toxicity / Trace elements / Transcriptome / Transcriptomes / Transcriptomics / Tree Biology