Journal Article

Evaluation of immunity and protective efficacy of a dengue-3 premembrane and envelope DNA vaccine in Aotus nancymae monkeys

Wu, Shuenn-Jue, Raviprakash, Kanakatte, Porter, Kevin R., Kochel, Tadeusz J., Guevara, Carolina, Blair, Patrick J., Salazar, Milagros, Olson, James G.

2006

Overview

A dengue (DEN) virus type 3 DNA vaccine expressing pre-membrane and envelope genes was tested for immunogenicity and protective efficacy in Aotus monkeys. Five of six vaccinated animals demonstrated moderate DEN-specific antibody responses as measured by ELISA and virus neutralization in vitro. By contrast, none of the six control animals developed detectable anti-DEN antibodies. When five vaccinated animals were challenged with live DEN-3 virus and viremia determined by PCR amplification of viral RNA in serum samples, one animal was completely protected and two were partially protected as indicated by a decrease in mean days of viremia. The results demonstrate the ability of the DEN-3 DNA vaccine to elicit a neutralizing antibody response and to partially protect against live virus challenge. These findings support the inclusion of this construct in a tetravalent DNA vaccine.

• Publisher: Elsevier Ltd,Elsevier
• Subject: Aotus / Aotus monkeys / Aotus nancymaee / Applied microbiology / Biological and medical sciences / Dengue / Dengue virus / DNA vaccine / Fundamental and applied biological sciences. Psychology / Microbiology / Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)