Journal Article

Recruitment of Antigen Presenting Cells to Skin Draining Lymph Node From HPV16E7-Expressing Skin Requires E7-Rb Interaction

Frazer, Ian H., Tuong, Zewen K., Teoh, Siok Min, Leggatt, Graham R., Kuo, Paula, Mattarollo, Stephen R.

2018

Overview

\"High-risk\" human papillomaviruses (HPV) infect keratinocytes of squamous epithelia. The HPV16E7 protein induces epithelial hyperplasia by binding Rb family proteins and disrupting cell cycle termination. Murine skin expressing HPV16E7 as a transgene from a keratin 14 promoter (K14.E7) demonstrates epithelial hyperplasia, dysfunctional antigen presenting cells, ineffective antigen presentation by keratinocytes, and production of immunoregulatory cytokines. Furthermore, grafted K14.E7 skin is not rejected from immunocompetent non-transgenic recipient animals. To establish the contributions of E7, of E7-Rb interaction and of epithelial hyperplasia to altered local skin immunity, K14.E7 skin was compared with skin from K14.E7 mice heterozygous for a mutant Rb unable to bind E7 (K14.E7xRb mice), that have normoplastic epithelium. Previously, we demonstrated that E7-speicfic T cells do not accumulate in K14.E7xRb skin grafts. Here, we further show that K14.E7xRb skin, like K14.E7 skin, is not rejected by immunocompetent non-transgenic animals. There were fewer CD11b antigen presenting cells in skin draining lymph nodes from animals recipient of K14.E7xRb grafts, when compared with animals receiving K14.E7 grafts or K5mOVA grafts. Maturation of migratory DCs derived from K14.E7xRb grafts found in the draining lymph nodes is significantly lower than that of K14.E7 grafts. Surprisingly, K14.E7xRb keratinocytes, unlike K14.E7 keratinocytes, are susceptible to E7 directed CTL-mediated lysis . We conclude that E7-Rb interaction and its associated epithelial hyperplasia partially contribute to the suppressive local immune responses in area affected by HPV16E7 expression.

• Publisher: Frontiers Media SA,Frontiers Media S.A
• Subject: Animals / Antigen presentation / Antigen-presenting cells / Antigen-Presenting Cells - immunology / Antigen-Presenting Cells - metabolism / Antigens / APC / CD11b antigen / Cell cycle / Cervical cancer / CIN / Cytotoxicity / Dendritic cells / Disease Models, Animal / Epidermis - immunology / Epidermis - pathology / Epidermis - transplantation / Epithelium / Female / HPV16 / Human papillomavirus / Human papillomavirus 16 - genetics / Human papillomavirus 16 - immunology / Humans / Hyperplasia / Hyperplasia - immunology / Hyperplasia - pathology / Immune response / Immunity (Disease) / Immunocompetence / Immunology / Immunoregulation / Keratin / Keratinocytes / Keratinocytes - immunology / Keratinocytes - metabolism / Lymph nodes / Lymph Nodes - cytology / Lymph Nodes - immunology / Lymphatic system / Lymphocytes / Lymphocytes T / Mice / Mice, Inbred C57BL / Mice, Transgenic / Papillomavirus E7 Proteins - genetics / Papillomavirus E7 Proteins - immunology / Papillomavirus E7 Proteins - metabolism / Papillomavirus Infections - immunology / Papillomavirus Infections - pathology / Plastic surgery / Protein Binding - genetics / Protein Binding - immunology / Proteins / Retinoblastoma Binding Proteins - genetics / Retinoblastoma Binding Proteins - immunology / Retinoblastoma Binding Proteins - metabolism / retinoblastoma protein / Retinoblastoma Protein - genetics / Retinoblastoma Protein - immunology / Retinoblastoma Protein - metabolism / Skin / Skin & tissue grafts / skin grafting / Transgenes / Transgenic animals