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Spelling Changes and Fluorescent Tagging With Prime Editing Vectors for Plants
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Journal Article
Spelling Changes and Fluorescent Tagging With Prime Editing Vectors for Plants
2021
Overview
Prime editing is an adaptation of the CRISPR-Cas system that uses a Cas9(H840A)-reverse transcriptase fusion and a guide RNA amended with template and primer binding site sequences to achieve RNA-templated conversion of the target DNA, allowing specified substitutions, insertions, and deletions. In the first report of prime editing in plants, a variety of edits in rice and wheat were described, including insertions up to 15 bp. Several studies in rice quickly followed, but none reported a larger insertion. Here, we report easy-to-use vectors for prime editing in dicots as well as monocots, their validation in
Nicotiana benthamiana
, rice, and Arabidopsis, and an insertion of 66 bp that enabled split-GFP fluorescent tagging.
Publisher
Frontiers Media SA,Frontiers Media S.A
Subject
