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Direct characterization of Blastocystis from faeces by PCR and evidence of zoonotic potential
by
KUMAR, S.
, MUNGTHIN, M.
, PARKAR, U.
, LEELAYOOVA, S.
, THOMPSON, R. C. A.
, TRAUB, R. J.
, VITALI, S.
, MORRIS, K.
in
Animals
/ Animals, Zoo - parasitology
/ Australia
/ Bacteria
/ Biological and medical sciences
/ Blastocystis
/ Blastocystis - classification
/ Blastocystis - genetics
/ Blastocystis - isolation & purification
/ Blastocystis Infections - parasitology
/ Blastocystis Infections - transmission
/ Cat Diseases - parasitology
/ Cat Diseases - transmission
/ Cats
/ Comparative studies
/ Culture
/ Culture Media
/ disease detection
/ Dog Diseases - parasitology
/ Dog Diseases - transmission
/ Dogs
/ feces
/ Feces - parasitology
/ Fundamental and applied biological sciences. Psychology
/ General aspects
/ General aspects and techniques. Study of several systematic groups. Models
/ Genes
/ genotype
/ Humans
/ in vitro amplification
/ Invertebrates
/ Marsupialia - parasitology
/ Methods
/ Microbiology
/ molecular characterization
/ molecular epidemiology
/ nucleotide sequences
/ pathogen identification
/ phylogeny
/ polymerase chain reaction
/ Polymerase Chain Reaction - methods
/ Primates - parasitology
/ ribosomal DNA
/ Sequence Analysis, DNA
/ Thailand
/ wild animals
/ zoo animals
/ Zoonoses
/ Zoonoses - parasitology
/ Zoonoses - transmission
2007
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Direct characterization of Blastocystis from faeces by PCR and evidence of zoonotic potential
by
KUMAR, S.
, MUNGTHIN, M.
, PARKAR, U.
, LEELAYOOVA, S.
, THOMPSON, R. C. A.
, TRAUB, R. J.
, VITALI, S.
, MORRIS, K.
in
Animals
/ Animals, Zoo - parasitology
/ Australia
/ Bacteria
/ Biological and medical sciences
/ Blastocystis
/ Blastocystis - classification
/ Blastocystis - genetics
/ Blastocystis - isolation & purification
/ Blastocystis Infections - parasitology
/ Blastocystis Infections - transmission
/ Cat Diseases - parasitology
/ Cat Diseases - transmission
/ Cats
/ Comparative studies
/ Culture
/ Culture Media
/ disease detection
/ Dog Diseases - parasitology
/ Dog Diseases - transmission
/ Dogs
/ feces
/ Feces - parasitology
/ Fundamental and applied biological sciences. Psychology
/ General aspects
/ General aspects and techniques. Study of several systematic groups. Models
/ Genes
/ genotype
/ Humans
/ in vitro amplification
/ Invertebrates
/ Marsupialia - parasitology
/ Methods
/ Microbiology
/ molecular characterization
/ molecular epidemiology
/ nucleotide sequences
/ pathogen identification
/ phylogeny
/ polymerase chain reaction
/ Polymerase Chain Reaction - methods
/ Primates - parasitology
/ ribosomal DNA
/ Sequence Analysis, DNA
/ Thailand
/ wild animals
/ zoo animals
/ Zoonoses
/ Zoonoses - parasitology
/ Zoonoses - transmission
2007
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Direct characterization of Blastocystis from faeces by PCR and evidence of zoonotic potential
by
KUMAR, S.
, MUNGTHIN, M.
, PARKAR, U.
, LEELAYOOVA, S.
, THOMPSON, R. C. A.
, TRAUB, R. J.
, VITALI, S.
, MORRIS, K.
in
Animals
/ Animals, Zoo - parasitology
/ Australia
/ Bacteria
/ Biological and medical sciences
/ Blastocystis
/ Blastocystis - classification
/ Blastocystis - genetics
/ Blastocystis - isolation & purification
/ Blastocystis Infections - parasitology
/ Blastocystis Infections - transmission
/ Cat Diseases - parasitology
/ Cat Diseases - transmission
/ Cats
/ Comparative studies
/ Culture
/ Culture Media
/ disease detection
/ Dog Diseases - parasitology
/ Dog Diseases - transmission
/ Dogs
/ feces
/ Feces - parasitology
/ Fundamental and applied biological sciences. Psychology
/ General aspects
/ General aspects and techniques. Study of several systematic groups. Models
/ Genes
/ genotype
/ Humans
/ in vitro amplification
/ Invertebrates
/ Marsupialia - parasitology
/ Methods
/ Microbiology
/ molecular characterization
/ molecular epidemiology
/ nucleotide sequences
/ pathogen identification
/ phylogeny
/ polymerase chain reaction
/ Polymerase Chain Reaction - methods
/ Primates - parasitology
/ ribosomal DNA
/ Sequence Analysis, DNA
/ Thailand
/ wild animals
/ zoo animals
/ Zoonoses
/ Zoonoses - parasitology
/ Zoonoses - transmission
2007
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Direct characterization of Blastocystis from faeces by PCR and evidence of zoonotic potential
Journal Article
Direct characterization of Blastocystis from faeces by PCR and evidence of zoonotic potential
2007
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Overview
In vitro propagation followed by PCR, and a PCR-based method capable of the direct detection of Blastocystis in faeces were utilized to detect Blastocystis from various hosts in Australia, including primates and their handlers from the Perth Zoo. In addition, Blastocystis isolates from dogs and humans living in a localized endemic community in Thailand were also characterized genetically. PCR-based detection directly from faeces was shown to be more sensitive compared with in vitro culture for the detection of Blastocystis. Moreover, phylogenetic analysis of Blastocystis isolates amplified utilizing in vitro techniques prior to PCR revealed that this method favoured the preferential amplification of Blastocystis subtype 5 over subtype 1. This study is the first to provide molecular-based evidence supporting the zoonotic potential of Blastocystis in dogs, possums and primates in a natural setting.
Publisher
Cambridge University Press
Subject
/ Bacteria
/ Biological and medical sciences
/ Blastocystis - classification
/ Blastocystis - isolation & purification
/ Blastocystis Infections - parasitology
/ Blastocystis Infections - transmission
/ Cats
/ Culture
/ Dogs
/ feces
/ Fundamental and applied biological sciences. Psychology
/ General aspects and techniques. Study of several systematic groups. Models
/ Genes
/ genotype
/ Humans
/ Methods
/ Polymerase Chain Reaction - methods
/ Thailand
/ Zoonoses
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