Journal Article

Longitudinal analysis of gene expression in porcine skeletal muscle after post-injection local injury

Uro-Coste, Emmanuelle, Régulations cellulaires: lipidoses et atherosclerose ; IFR 31 Louis Bugnard (IFR 31) ; Université Toulouse III - Paul Sabatier (UT3) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Concordet, Didier, Tosser-Klopp, Gwenola, Physiologie et Toxicologie Expérimentales ; Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de To

2007

Overview

PURPOSE: The purpose of this study is to describe the time course of gene expression in a skeletal muscle local injury induced by an intramuscular (IM) injection, and to compare the dynamics of gene expression with pathological events. MATERIALS AND METHODS: Ten piglets received 4 IM injections of propylene glycol in the longissimus dorsi muscles 6 h, 2, 7, and 21 days before euthanasia, where control and injected muscle sites were sampled for RNA isolation and microscopic examination. The hybridization of nylon cDNA microarrays was carried out with radioactive probes obtained from the muscle RNA. RESULTS: 153 genes were found under- or over-expressed at least once among the investigated time-conditions. The eight most discriminant genes were also identified: Two genes (GTP-binding protein RAD and Ankyrin repeat domain protein) were over-expressed at 6 h and six genes between 2 and 21 days (Osteonectin, Fibronectin, Matrix metalloproteinase-2, Collagen alpha 1(I) chain, Collagen alpha 2(I) chain, and Thymosin beta-4). Necrosis, inflammation and regeneration were observed through both the dynamics of gene expression profiles and through the microscopic examinations. CONCLUSION: Our data demonstrate that several pathways are involved in post-injection muscle injury, and that necrosis, inflammation and regeneration are not sequential but occur in parallel.

• Publisher: CCSD,American Association of Pharmaceutical Scientists,Springer,Springer Nature B.V
• Subject: Animals / Biological and medical sciences / Collagen / Collagen - genetics / Collagen Type I / Collagen Type I - genetics / Down-Regulation / Down-Regulation - genetics / Fibronectins / Fibronectins - genetics / Gene expression / Gene Expression Profiling / General pharmacology / Hogs / Injections / Injections, Intramuscular / Injections, Intramuscular - adverse effects / Life Sciences / Male / Matrix Metalloproteinase 2 / Matrix Metalloproteinase 2 - genetics / Medical sciences / Muscle Proteins / Muscle Proteins - genetics / Muscle, Skeletal / Muscle, Skeletal - injuries / Muscle, Skeletal - metabolism / Muscular system / Oligonucleotide Array Sequence Analysis / Oligonucleotide Array Sequence Analysis - methods / Osteonectin / Osteonectin - genetics / Pharmaceutical technology. Pharmaceutical industry / Pharmaceuticals / Pharmacology. Drug treatments / ras Proteins / ras Proteins - genetics / Swine / Thymosin / Thymosin - genetics / Time Factors / Up-Regulation / Up-Regulation - genetics
• ISBN: 0002474729000