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Nanoenviroments of the β-Subunit of L-Type Voltage-Gated Calcium Channels in Adult Cardiomyocytes
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Nanoenviroments of the β-Subunit of L-Type Voltage-Gated Calcium Channels in Adult Cardiomyocytes
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Journal Article
Nanoenviroments of the β-Subunit of L-Type Voltage-Gated Calcium Channels in Adult Cardiomyocytes
2022
Overview
In cardiomyocytes, Ca 2+ influx through L-type voltage-gated calcium channels (LTCCs) following membrane depolarization regulates crucial Ca 2+ -dependent processes including duration and amplitude of the action potentials and excitation-contraction coupling. LTCCs are heteromultimeric proteins composed of the Ca v α 1 , Ca v β, Ca v α 2 δ and Ca v γ subunits. Here, using ascorbate peroxidase (APEX2)-mediated proximity labeling and quantitative proteomics, we identified 61 proteins in the nanoenvironments of Ca v β 2 in cardiomyocytes. These proteins are involved in diverse cellular functions such as cellular trafficking, cardiac contraction, sarcomere organization and excitation-contraction coupling. Moreover, pull-down assays and co-immunoprecipitation analyses revealed that Ca v β 2 interacts with the ryanodine receptor 2 (RyR2) in adult cardiomyocytes, probably coupling LTCCs and the RyR2 into a supramolecular complex at the dyads. This interaction is mediated by the Src-homology 3 domain of Ca v β 2 and is necessary for an effective pacing frequency-dependent increase of the Ca 2+ -induced Ca 2+ release mechanism in cardiomyocytes.
Publisher
Frontiers Media SA,Frontiers Media S.A
