Asset Details
Do you wish to reserve the book?
Vitamin K supports TGF-β1 depended in vitro human Langerhans cell differentiation and function via Axl
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Vitamin K supports TGF-β1 depended in vitro human Langerhans cell differentiation and function via Axl
Do you wish to request the book?
Vitamin K supports TGF-β1 depended in vitro human Langerhans cell differentiation and function via Axl
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Vitamin K supports TGF-β1 depended in vitro human Langerhans cell differentiation and function via Axl
2025
Overview
On the outermost edge of the body a dense network of dendritic cells (DCs), the so-called Langerhans cells (LCs), represents the first immune barrier. The establishment and maintenance of this epidermal network is dependent on the cytokine transforming growth factor-β1 (TGF-β1) expressed by keratinocytes (KC) and LCs. We recently identified a crucial downstream effector of TGF-β1, the receptor tyrosine kinase Axl. Axl belongs to the TAM receptor family, which also includes Tyro3 and Mer, and is activated through the vitamin K-dependent ligands Gas6 and Protein S.
We have now established that TGF-β1 dependent
human LC generation from CD34
progenitor cells can be enhanced by Axl over-expression.
Additionally, we supplemented vitamin K into serum-free human LC generation cultures in order to activate the endogenous ligands Gas6 and Protein S. Vitamin K exhibited supportive effects on LC differentiation and LC-associated gene expression. The vitamin K antagonist warfarin on the other hand, hindered efficient LC differentiation. Blocking antibodies against Axl abrogated the positive effect of vitamin K on LC differentiation. Lastly, vitamin K downregulated the immune activation marker CD86 during LC differentiation and blocked the upregulation of CD86 during LC activation
, in an Axl independent manner.
Taken together, we provide evidence for the supportive role of vitamin K in regulating skin immunity.
Publisher
Frontiers Media SA,Frontiers Media S.A
Subject
/ Axl Receptor Tyrosine Kinase
/ Cell Differentiation - drug effects
/ Graphs
/ Humans
/ Intercellular Signaling Peptides and Proteins - metabolism
/ Kinases
/ Langerhans Cells - drug effects
/ Langerhans Cells - immunology
/ Langerhans Cells - metabolism
/ Ligands
/ Protein-tyrosine kinase receptors
/ Proteins
/ Proto-Oncogene Proteins - genetics
/ Proto-Oncogene Proteins - metabolism
/ Receptor Protein-Tyrosine Kinases - genetics
/ Receptor Protein-Tyrosine Kinases - metabolism
/ receptor tyrosine kinase Axl
/ Transforming Growth Factor beta1 - metabolism
