Journal Article

Isolation and Characterization of Diuron-degrading Bacteria from Lotic Surface Water

Batisson, Isabelle, Sancelme, Martine, Pesce, Stéphane, Besse-Hoggan, Pascale, Bohatier, Jacques

2007

Overview

The bacterial community structure of a diuron-degrading enrichment culture from lotic surface water samples was analyzed and the diuron-degrading strains were selected using a series of techniques combining temporal temperature gradient gel electrophoresis (TTGE) of 16 S rDNA gene V1-V3 variable regions, isolation of strains on agar plates, colony hybridization methods, and biodegradation assays. The TTGE fingerprints revealed that diuron had a strong impact on bacterial community structure and highlighted both diuron-sensitive and diuron-adapted bacterial strains. Two bacterial strains, designated IB78 and IB93 and identified as belonging to Pseudomonas sp. and Stenotrophomonas sp., were isolated and shown to degrade diuron in pure resting cells in a first-order kinetic reaction during the first 24 h of incubation with no 3,4-DCA detected. The percentages of degradation varied from 25% to 60% for IB78 and 20% to 65% for IB93 and for a diuron concentration range from 20 mg/L to 2 mg/L, respectively. It is interesting to note that diuron was less degraded by single isolates than by mixed resting cells, thereby underlining a cumulative effect between these two strains. To the best of our knowledge, this is the first report of diuron-degrading strains isolated from lotic surface water.

• Publisher: New York : Springer-Verlag,Springer Science + Business Media, Inc,Springer,Springer Nature B.V,Springer Verlag
• Subject: Agricultural soils / analysis / Bacteria / bacterial communities / Biodegradation / Biodegradation, Environmental / Biological and medical sciences / Cell culture / classification / Community structure / Cultural enrichment / Culture Media / denaturing gradient gel electrophoresis / Diuron / Diuron - metabolism / DNA, Bacterial / DNA, Bacterial - analysis / DNA, Bacterial - isolation & purification / Ecotoxicology / Electrophoresis / Electrophoresis, Polyacrylamide Gel / Electrophoresis, Polyacrylamide Gel - methods / enrichment culture / Enrichment media / Flasks / Fundamental and applied biological sciences. Psychology / Gel electrophoresis / Gels / genes / genetics / Herbicides / Herbicides - metabolism / Hybridization / isolation & purification / Life Sciences / lotic systems / metabolism / methods / Microbiological strains / Microbiology / Molecular Sequence Data / Phylogeny / Pseudomonas / Pseudomonas - classification / Pseudomonas - genetics / Pseudomonas - isolation & purification / Ribosomal DNA / Rivers / Rivers - microbiology / RNA, Ribosomal, 16S / RNA, Ribosomal, 16S - genetics / Sequence Analysis, DNA / Soil pollution / Stenotrophomonas / Stenotrophomonas - classification / Stenotrophomonas - genetics / Stenotrophomonas - isolation & purification / Strains (organisms) / Surface water / Temperature gradients / Toxicology / Water analysis / Water Pollutants / Water Pollutants - metabolism / Water sampling