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High-contrast, synchronous volumetric imaging with selective volume illumination microscopy
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Journal Article
High-contrast, synchronous volumetric imaging with selective volume illumination microscopy
2020
Overview
Light-field fluorescence microscopy uniquely provides fast, synchronous volumetric imaging by capturing an extended volume in one snapshot, but often suffers from low contrast due to the background signal generated by its wide-field illumination strategy. We implemented light-field-based selective volume illumination microscopy (SVIM), where illumination is confined to only the volume of interest, removing the background generated from the extraneous sample volume, and dramatically enhancing the image contrast. We demonstrate the capabilities of SVIM by capturing cellular-resolution 3D movies of flowing bacteria in seawater as they colonize their squid symbiotic partner, as well as of the beating heart and brain-wide neural activity in larval zebrafish. These applications demonstrate the breadth of imaging applications that we envision SVIM will enable, in capturing tissue-scale 3D dynamic biological systems at single-cell resolution, fast volumetric rates, and high contrast to reveal the underlying biology.
Thai Truong et al. present light-field-based selective volume illumination microscopy (SVIM), a method for enhancing image contrast and resolution by combining light-field microscopy and selective plane illumination microscopy. They generate cellular-resolution 3D movies by applying SVIM to flowing bacteria in seawater and to the beating heart and whole brain of larval zebrafish.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ 14/63
/ 14/69
/ Animals
/ Bacteria
/ Biology
/ Biomedical and Life Sciences
/ Decapodiformes - microbiology
/ Decapodiformes - ultrastructure
/ Host Microbial Interactions - physiology
/ Image Processing, Computer-Assisted - instrumentation
/ Image Processing, Computer-Assisted - methods
/ Imaging, Three-Dimensional - instrumentation
/ Imaging, Three-Dimensional - methods
/ Larva
/ Light
/ Microscopy, Fluorescence - instrumentation
/ Microscopy, Fluorescence - methods
