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Correlation between polymerase chain reaction analysis of the histidine biosynthesis operon, randomly amplified polymorphic DNA analysis and phenotypic characterization of dairy Lactococcus isolates
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Correlation between polymerase chain reaction analysis of the histidine biosynthesis operon, randomly amplified polymorphic DNA analysis and phenotypic characterization of dairy Lactococcus isolates
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Correlation between polymerase chain reaction analysis of the histidine biosynthesis operon, randomly amplified polymorphic DNA analysis and phenotypic characterization of dairy Lactococcus isolates
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Journal Article
Correlation between polymerase chain reaction analysis of the histidine biosynthesis operon, randomly amplified polymorphic DNA analysis and phenotypic characterization of dairy Lactococcus isolates
1999
Overview
A collection of 32 lactococcal strains isolated from raw milk in the Camembert RDO (registered designation of origin) area were phenotypically and genotypically characterized. As expected for environmental isolates, all strains had a Lactococcus lactis subsp. lactis phenotype. The strains were then genotypically identified by the randomly amplified polymorphic DNA (RAPD) technique, using reference strains of lactococci. Two major clusters were identified containing the two subspecies lactis and cremoris. The subspecies lactis cluster could be divided into five subgroups whereas there was a high coefficient of similarity between all strains in the subspecies cremoris cluster. This RAPD classification was then compared with that of a traditional PCR assay using L. lactis species-specific primers corresponding to part of the histidine biosynthesis operon. The two subspecies were differentiated by the size of the fragment amplified (about 200 bp longer for subspecies cremoris). Unlike preliminary phenotypic assignments, the results of PCR experiments corroborated the genotypic identification of the lactococcal strains by RAPD allowing the technique to be reconsidered on the basis of its taxonomic efficiency.
Publisher
Springer,Springer Nature B.V,Springer Verlag
Subject
/ Bacteria
/ Bacteriological methods and techniques used in bacteriology
/ Biological and medical sciences
/ Clusters
/ DNA
/ Fundamental and applied biological sciences. Psychology
/ genetics
/ Genotype
/ Lactococcus - classification
/ Lactococcus lactis - classification
/ Lactococcus lactis - genetics
/ Lactococcus lactis subsp. lactis
/ Microbiology and Parasitology
/ Milk
/ Operon
/ Random amplified polymorphic DNA
/ Random Amplified Polymorphic DNA Technique
/ Random Amplified Polymorphic DNA Technique - classification
/ raw milk
