Journal Article

Structure of coronavirus main proteinase reveals combination of a chymotrypsin fold with an extra α-helical domain

Siddell, Stuart G., Anand, Kanchan, Ziebuhr, John, Mesters, Jeroen R., Palm, Gottfried J., Hilgenfeld, Rolf

2002

Overview

The key enzyme in coronavirus polyprotein processing is the viral main proteinase, M pro , a protein with extremely low sequence similarity to other viral and cellular proteinases. Here, the crystal structure of the 33.1 kDa transmissible gastroenteritis (corona)virus M pro is reported. The structure was refined to 1.96 Å resolution and revealed three dimers in the asymmetric unit. The mutual arrangement of the protomers in each of the dimers suggests that M pro self‐processing occurs in trans . The active site, comprised of Cys144 and His41, is part of a chymotrypsin‐like fold that is connected by a 16 residue loop to an extra domain featuring a novel α‐helical fold. Molecular modelling and mutagenesis data implicate the loop in substrate binding and elucidate S1 and S2 subsites suitable to accommodate the side chains of the P1 glutamine and P2 leucine residues of M pro substrates. Interactions involving the N‐terminus and the α‐helical domain stabilize the loop in the orientation required for trans ‐cleavage activity. The study illustrates that RNA viruses have evolved unprecedented variations of the classical chymotrypsin fold.

• Publisher: John Wiley & Sons, Ltd,Nature Publishing Group UK,Oxford University Press
• Subject: 3C-like / Amino Acid Sequence / Binding Sites / catalytic dyad / Chymotrypsin - chemistry / coronavirus / Crystallography, X-Ray / Cysteine Endopeptidases - chemistry / Cysteine Endopeptidases - genetics / Dimerization / EMBO23 / EMBO40 / Models, Molecular / Molecular Sequence Data / Mutagenesis / Protein Binding / Protein Conformation / Protein Folding / Protein Structure, Tertiary / proteinase / Recombinant Fusion Proteins - chemistry / Sequence Alignment / Sequence Homology, Amino Acid / Transmissible gastroenteritis virus - enzymology / Transmissible gastroenteritis virus - genetics / X-ray crystallography