Journal Article

The Ectomycorrhizal Fungus Laccaria bicolor Produces Lipochitooligosaccharides and Uses the Common Symbiosis Pathway to Colonize Populus Roots

Ané, Jean-Michel, Schnell, Kimberly, Maillet, Fabienne, Wang, Yunqian, Strauss, Steven, University of Wisconsin-Madison, Laboratoire de Recherche en Sciences Végétales (LRSV) ; Université Toulouse III - Paul Sabatier (UT3) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Garcia, Kevin, Schleif, Nathaniel, Venkateshwaran, Muthusubramanian, Irving, Thomas, United States Department of Agriculture (USDA) : 2017-67014-26530 WIS01695 ; National Science Foundation (NSF) : DGE-1256259 ; United States Department of Energy (DOE) : DE-SC0018247 ; National Science Foundation (NSF) : 1546742, 1331098 ; Tree Genomics and Biosafety Cooperative at Oregon State University ; NSF Center for Advanced Forestry Systems : 1238305 ; Plant-Microbe Interfaces Scientific Focus Area in the Genomic Science Program ; Office of Biological and Environmental Research in the DOE ; Office of Science ; United States Department of Energy (DOE) : DE-AC05-00OR22725, ANR-11-INBS-0010,MET

2019

Overview

Mycorrhizal fungi form mutualistic associations with the roots of most land plants and provide them with mineral nutrients from the soil in exchange for fixed carbon derived from photosynthesis. The common symbiosis pathway (CSP) is a conserved molecular signaling pathway in all plants capable of associating with arbuscular mycorrhizal fungi. It is required not only for arbuscular mycorrhizal symbiosis but also for rhizobia-legume and actinorhizal symbioses. Given its role in such diverse symbiotic associations, we hypothesized that the CSP also plays a role in ectomycorrhizal associations. We showed that the ectomycorrhizal fungus Laccaria bicolor produces an array of lipochitooligosaccharides (LCOs) that can trigger both root hair branching in legumes and, most importantly, calcium spiking in the host plant Populus in a CASTOR/POLLUX-dependent manner. Nonsulfated LCOs enhanced lateral root development in Populus in a calcium/calmodulin-dependent protein kinase (CCaMK)-dependent manner, and sulfated LCOs enhanced the colonization of Populus by L. bicolor. Compared with the wildtype Populus, the colonization of CASTOR/POLLUX and CCaMK RNA interference lines by L. bicolor was reduced. Our work demonstrates that similar to other root symbioses, L. bicolor uses the CSP for the full establishment of its mutualistic association with Populus.

• Publisher: American Society of Plant Biologists (ASPB),CCSD,American Society of Plant Biologists
• Subject: BASIC BIOLOGICAL SCIENCES / Botanics / Calcium - metabolism / Calcium Channels - metabolism / Calcium-Calmodulin-Dependent Protein Kinases - genetics / Calcium-Calmodulin-Dependent Protein Kinases - metabolism / Ecology, environment / Gene Expression Regulation, Plant / Laccaria - metabolism / Life Sciences / Lipopolysaccharides - chemistry / Lipopolysaccharides - metabolism / Microbiology and Parasitology / Mycology / Mycorrhizae - growth & development / Mycorrhizae - metabolism / Mycorrhizae - physiology / Plant Roots - chemistry / Plant Roots - growth & development / Plant Roots - metabolism / Plant Roots - microbiology / Plants, Genetically Modified / Populus - genetics / Populus - metabolism / Signal Transduction / Symbiosis / Symbiosis - physiology / Vegetal Biology
• ISBN: 0004891622000