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Tn7 transposition regulator TnsC interacts with the transposase subunit TnsB and target selector TnsD
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Tn7 transposition regulator TnsC interacts with the transposase subunit TnsB and target selector TnsD
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Tn7 transposition regulator TnsC interacts with the transposase subunit TnsB and target selector TnsD
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Journal Article
Tn7 transposition regulator TnsC interacts with the transposase subunit TnsB and target selector TnsD
2014
Overview
The excision of transposon Tn7 from a donor site and its insertion into its preferred target site, attachment site attTn7 , is mediated by four Tn7 -encoded transposition proteins: TnsA, TnsB, TnsC, and TnsD. Transposition requires the assembly of a nucleoprotein complex containing all four Tns proteins and the DNA substrates, the donor site containing Tn7 , and the preferred target site attTn7 . TnsA and TnsB together form the heteromeric Tn7 transposase, and TnsD is a target-selecting protein that binds specifically to attTn7 . TnsC is the key regulator of transposition, interacting with both the TnsAB transposase and TnsD- attTn7 . We show here that TnsC interacts directly with TnsB, and identify the specific region of TnsC involved in the TnsB–TnsC interaction during transposition. We also show that a TnsC mutant defective in interaction with TnsB is defective for Tn7 transposition both in vitro and in vivo. Tn7 displays cis -acting target immunity, which blocks Tn7 insertion into a target DNA that already contains Tn7. We provide evidence that the direct TnsB–TnsC interaction that we have identified also mediates cis -acting Tn7 target immunity. We also show that TnsC interacts directly with the target selector protein TnsD.
Publisher
National Academy of Sciences,National Acad Sciences
Subject
Attachment Sites, Microbiological - physiology
/ DNA
/ DNA Transposable Elements - genetics
/ DNA-Binding Proteins - genetics
/ DNA-Binding Proteins - metabolism
/ Escherichia coli - metabolism
/ Escherichia coli Proteins - genetics
/ Escherichia coli Proteins - metabolism
/ immunity
/ mutants
/ Proteins
