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In vivo three-photon imaging of activity of GCaMP6-labeled neurons deep in intact mouse brain
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In vivo three-photon imaging of activity of GCaMP6-labeled neurons deep in intact mouse brain
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In vivo three-photon imaging of activity of GCaMP6-labeled neurons deep in intact mouse brain
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Journal Article
In vivo three-photon imaging of activity of GCaMP6-labeled neurons deep in intact mouse brain
2017
Overview
Ouzounov
et al
. report calcium imaging with three-photon microscopy in the mouse brain. The approach enabled noninvasive recording of activity with high spatial and temporal resolution from GCaMP6-labeled neurons located as deep as the hippocampus.
High-resolution optical imaging is critical to understanding brain function. We demonstrate that three-photon microscopy at 1,300-nm excitation enables functional imaging of GCaMP6s-labeled neurons beyond the depth limit of two-photon microscopy. We record spontaneous activity from up to 150 neurons in the hippocampal stratum pyramidale at ∼1-mm depth within an intact mouse brain. Our method creates opportunities for noninvasive recording of neuronal activity with high spatial and temporal resolution deep within scattering brain tissues.
Publisher
Nature Publishing Group US,Nature Publishing Group
Subject
/ 42/35
/ 631/378
/ 64/60
/ Animals
/ Biomedical Engineering/Biotechnology
/ Brain
/ Green Fluorescent Proteins - analysis
/ Green Fluorescent Proteins - genetics
/ Green Fluorescent Proteins - metabolism
/ Male
/ Microscopy, Fluorescence, Multiphoton - methods
/ Neurons
/ Recombinant Fusion Proteins - analysis
/ Recombinant Fusion Proteins - metabolism
/ Recombinant Proteins - analysis
/ Recombinant Proteins - genetics
/ Recombinant Proteins - metabolism
/ Rodents
