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Molecular characterization of TaSTOP1 homoeologues and their response to aluminium and proton (H+) toxicity in bread wheat (Triticum aestivumL.)
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Molecular characterization of TaSTOP1 homoeologues and their response to aluminium and proton (H+) toxicity in bread wheat (Triticum aestivumL.)
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Molecular characterization of TaSTOP1 homoeologues and their response to aluminium and proton (H+) toxicity in bread wheat (Triticum aestivumL.)
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Journal Article
Molecular characterization of TaSTOP1 homoeologues and their response to aluminium and proton (H+) toxicity in bread wheat (Triticum aestivumL.)
2013
Overview
Background
Aluminium (Al) toxicity is considered to be one of the major constraints affecting crop productivity on acid soils. Being a trait governed by multiple genes, the identification and characterization of novel transcription factors (TFs) regulating the expression of entire response networks is a very promising approach. Therefore, the aim of the present study was to clone, localize, and characterize the
TaSTOP1
gene, which belongs to the zinc finger family (Cys2His2 type) transcription factor, at molecular level in bread wheat.
Results
TaSTOP1
loci were cloned and localized on the long arm of homoeologous group 3 chromosomes [3AL (
TaSTOP1-A
), 3BL (
TaSTOP1-B
) and 3DL (
TaSTOP1-D
)] in bread wheat. TaSTOP1 showed four potential zinc finger domains and the homoeologue TaSTOP1-A exhibited transactivation activity in yeast. Expression profiling of
TaSTOP1
transcripts identified the predominance of homoeologue
TaSTOP1-A
followed by
TaSTOP1-D
over
TaSTOP1-B
in root and only predominance of
TaSTOP1-A
in shoot tissues of two diverse bread wheat genotypes. Al and proton (H
+
) stress appeared to slightly modulate the transcript of
TaSTOP1
homoeologues expression in both genotypes of bread wheat.
Conclusions
Physical localization of
TaSTOP1
results indicated the presence of a single copy of
TaSTOP1
on homoeologous group 3 chromosomes in bread wheat. The three homoeologues of
TaSTOP1
have similar genomic structures, but showed biased transcript expression and different response to Al and proton (H
+
) toxicity. These results indicate that
TaSTOP1
homoeologues may differentially contribute under Al or proton (H
+
) toxicity in bread wheat. Moreover, it seems that
TaSTOP1-A
transactivation potential is constitutive and may not depend on the presence/absence of Al at least in yeast. Finally, the localization of
TaSTOP1
on long arm of homoeologous group 3 chromosomes and the previously reported major loci associated with Al resistance at chromosome 3BL, through QTL and genome wide association mapping studies suggests that
TaSTOP1
could be a potential candidate gene for genomic assisted breeding for Al tolerance in bread wheat.
Publisher
BioMed Central
Subject
/ aluminum
/ Biomedical and Life Sciences
/ breeding
/ Chromosomes, Plant - genetics
/ genes
/ Genetics and crop biotechnology
/ genotype
/ loci
/ Protons
/ tissues
/ toxicity
/ Triticum
/ wheat
/ yeasts
