Journal Article

Single-molecule investigations of single-chain cellulose biosynthesis

Górniak, Ireneusz, Brady, Sonia K., Lang, Matthew J., Hilton, Mark A., Manning, Harris W., Zimmer, Jochen, Johnson, Madeline M.

2022

Overview

Cellulose biosynthesis in sessile bacterial colonies originates in the membrane-integrated bacterial cellulose synthase (Bcs) AB complex. We utilize optical tweezers to measure single-strand cellulose biosynthesis by BcsAB from Rhodobacter sphaeroides. Synthesis depends on uridine diphosphate glucose, Mg2+, and cyclic diguanosine monophosphate, with the last displaying a retention time of ~80 min. Below a stall force of 12.7 pN, biosynthesis is relatively insensitive to force and proceeds at a rate of one glucose addition every 2.5 s at room temperature, increasing to two additions per second at 37°. At low forces, conformational hopping is observed. Single-strand cellulose stretching unveiled a persistence length of 6.2 nm, an axial stiffness of 40.7 pN, and an ability for complexes to maintain a tight grip, with forces nearing 100 pN. Stretching experiments exhibited hysteresis, suggesting that cellulose microstructure underpinning robust biofilms begins to form during synthesis. Cellohexaose spontaneously binds to nascent single cellulose strands, impacting polymer mechanical properties and increasing BcsAB activity.

• Publisher: National Academy of Sciences,Proceedings of the National Academy of Sciences
• Subject: BASIC BIOLOGICAL SCIENCES / Biofilms / Biological Sciences / Biophysics and Computational Biology / Biosynthesis / Carbohydrate Metabolism / Cellulose / Cellulose - metabolism / Cellulose synthase / Glucose / Glucose - metabolism / Magnesium / Mechanical properties / optical tweezers cellulose / Polymers / Retention time / Rhodobacter sphaeroides - metabolism / Room temperature / Sessile species / single molecule studies / Stiffness / Stretching / Uridine / Uridine Diphosphate Glucose - metabolism