Journal Article

Planomonospora algeriensis sp. nov., an actinobacterium isolated from a Saharan soil of Algeria

Bouznada, Khaoula, Klenk, Hans-Peter, Mokrane, Salim, Sabaou, Nasserdine, Bouras, Noureddine, Zitouni, Abdelghani, Chaabane Chaouch, Fawzia, Pötter, Gabriele, Spröer, Cathrin

2017

Overview

A filamentous actinobacterium, designated strain PM3 T , was isolated from a Saharan soil sample collected from Béni-Abbès, Béchar (South-West Algeria). A polyphasic taxonomic study was carried out to establish the status of strain PM3 T . The isolate was found to have morphological and chemotaxonomical properties associated with members of the genus Planomonospora . The new isolated microorganism developed cylindrical sporangia arranged in double parallel rows on aerial mycelium, each one containing a motile single sporangiospore. The cell wall of the strain was found to contain meso -diaminopimelic acid. Whole-cell hydrolysates were found to contain madurose, glucose, mannose and ribose. The predominant menaquinone was identified as MK-9(H 2 ) (69.6%). The polar lipids detected were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylethanolamine, phosphatidylhydroxyethanolamine and glucosamine-containing lipids. The major fatty acids were found to be C 17:1 ω9c (38.6%) and C 17:0 (24.2%). Results of 16S rRNA gene sequence comparison revealed that strain PM3 T shared a high degree of 16S rRNA gene sequence similarity with Planomonospora sphaerica DSM 44632 T (99.3%), Planomonospora parontospora subsp. parontospora DSM 43177 T (99.2%) and P. parontospora subsp. antibiotica DSM 43869 T (99.0%). DNA–DNA hybridization values between strain PM3 T and the type strains of the closely related species were between 58.4 and 70.1%. The combination of phylogenetic analysis, DNA–DNA relatedness data, phenotypic characteristics and chemotaxonomic data support the conclusion that strain PM3 T represents a novel species of the genus Planomonospora , for which the name Planomonospora algeriensis sp. nov. is proposed. The type strain is PM3 T (=DSM 46752 T  = CECT 9047 T ).

• Publisher: Springer International Publishing,Springer Nature B.V
• Subject: Actinobacteria - classification / Actinobacteria - genetics / Algeria / Bacteria / Biomedical and Life Sciences / cell walls / chemotaxonomy / Deoxyribonucleic acid / DNA / genus / glucose / Life Sciences / Lipids / mannose / Medical Microbiology / menaquinones / Microbiology / mycelium / nucleic acid hybridization / nucleotide sequences / Original Paper / phenotype / phosphatidylethanolamines / Phylogeny / Planomonospora parontospora / Planomonospora sphaerica / Plant Sciences / ribose / RNA, Ribosomal, 16S - genetics / sequence homology / soil / Soil Microbiology / Soil microorganisms / Soil Science & Conservation / species / sporangia / Taxonomy