Journal Article

Association of miRNA targetome variants in LAMC1 and GNB3 genes with colorectal cancer and obesity

Gholami, Morteza, Amoli, Mahsa M., Larijani, Bagher, Zoughi, Marziyeh, Hasani‐Ranjbar, Shirin, Behboo, Roobic, Taslimi, Reza, Bastami, Milad, Kazemeini, Alireza

2022

Overview

Background Colorectal cancer (CRC) is one of the most common obesity‐associated cancers. Inflammation is also considered the most important factor between obesity and CRC. This study aimed to examine miRNAs binding sites variants on inflammatory genes identified using bioinformatics and systematic approach on clinical samples that were collected from CRC patients and controls. Methods The candidate variants related to CRC inflammatory genes were obtained from genome‐wide association studies and their population‐specific haplotypes. The variants were analyzed according to their genomic position on the miRNA targetome. Targetome variants in inflammation‐related genes were selected for genetic association study by TaqMan genotyping assay. Results The GG genotype of rs7473 decreased the risk of obesity (p < 0.05). Heterozygous genotype (GA) of rs1547715 decreased the risk of CRC (p < 0.05). In the rs7473/rs1547715 genotype and haplotype, the frequencies of AA/GA and GG/AA lessened in CRC and obesity, respectively (p < 0.05). Conclusions The variants of rs7473 and rs1547715 were associated with obesity and CRC, respectively. The above‐mentioned associations could be made based on the interactions of these variants with miRNAs. This study aimed to examine miRNAs binding sites variants on inflammatory genes identified using bioinformatics and systematic approach on clinical samples collected from CRC patients and controls. Targetome variants in inflammation‐related genes were selected for genetic association study by TaqMan genotyping assay. The variants of rs1547715 and rs7473 are associated with CRC and obesity, respectively. These associations may be based on the interactions of these variants with miRNAs.

• Publisher: John Wiley & Sons, Inc,John Wiley and Sons Inc,Wiley
• Subject: Binding sites / Bioinformatics / Body mass index / cancer biology / Case-Control Studies / Colorectal cancer / Colorectal Neoplasms - genetics / Disease / Family medical history / Gene expression / Genetic Predisposition to Disease / genetic variants / Genome-Wide Association Study / Genotype / Genotypes / Genotyping / Haplotypes / Heterotrimeric GTP-Binding Proteins - genetics / Hospitals / Humans / Inflammation / Informed consent / Laminin - genetics / MicroRNAs / MicroRNAs - genetics / miRNA / Obesity / Obesity - genetics / Overweight / pathway analysis / Polymorphism, Single Nucleotide / Population studies