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A Sanger/Pyrosequencing Hybrid Approach for the Generation of High-Quality Draft Assemblies of Marine Microbial Genomes

by Friedman, Robert , Feldblyum, Tamara , Goldberg, Susanne M. D. , Venter, J. Craig , Thomas, Torsten , Busam, Dana , Johnson, Justin , Sutton, Granger , Li, Kelvin , Ferriera, Steve , Halpern, Aaron , Kravitz, Saul A. , Khouri, Hoda , Lauro, Federico M. , Rogers, Yu-Hui , Tallon, Luke , Venter, Eli , Frazier, Marvin , Strausberg, Robert

in Biochemistry / Biological Sciences / Biotechnology - methods / Biotechnology - trends / Computational Biology - methods / Contig Mapping / Emerging technology / Genes, Bacterial / Genome size / Genome, Bacterial / Genomes / Genomics / Hybridity / Libraries / Methods / Microorganisms / Organisms / Plasmids / Scaffolds / Sequence Analysis, DNA - methods / Sequencing

2006

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A Sanger/Pyrosequencing Hybrid Approach for the Generation of High-Quality Draft Assemblies of Marine Microbial Genomes

2006

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A Sanger/Pyrosequencing Hybrid Approach for the Generation of High-Quality Draft Assemblies of Marine Microbial Genomes

2006

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Journal Article

A Sanger/Pyrosequencing Hybrid Approach for the Generation of High-Quality Draft Assemblies of Marine Microbial Genomes

Friedman, Robert,

Feldblyum, Tamara,

Goldberg, Susanne M. D.,

Venter, J. Craig,

Thomas, Torsten,

Busam, Dana,

Johnson, Justin,

Sutton, Granger,

Li, Kelvin,

Ferriera, Steve,

Halpern, Aaron,

Kravitz, Saul A.,

Khouri, Hoda,

Lauro, Federico M.,

Rogers, Yu-Hui,

Tallon, Luke,

Venter, Eli,

Frazier, Marvin,

Strausberg, Robert

2006

Overview

Since its introduction a decade ago, whole-genome shotgun sequencing (WGS) has been the main approach for producing cost-effective and high-quality genome sequence data. Until now, the Sanger sequencing technology that has served as a platform for WGS has not been truly challenged by emerging technologies. The recent introduction of the pyrosequencing-based 454 sequencing platform (454 Life Sciences, Branford, CT) offers a very promising sequencing technology alternative for incorporation in WGS. In this study, we evaluated the utility and cost-effectiveness of a hybrid sequencing approach using 3730x/ Sanger data and 454 data to generate higher-quality lower-cost assemblies of microbial genomes compared to current Sanger sequencing strategies alone.

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Publisher

National Academy of Sciences,National Acad Sciences

Subject

Biochemistry

/ Biological Sciences

/ Biotechnology - methods

/ Biotechnology - trends

/ Computational Biology - methods

/ Contig Mapping

/ Emerging technology