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Liquid Matrix Deposition on Conductive Hydrophobic Surfaces for Tuning and Quantitation in UV-MALDI Mass Spectrometry
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Liquid Matrix Deposition on Conductive Hydrophobic Surfaces for Tuning and Quantitation in UV-MALDI Mass Spectrometry
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Liquid Matrix Deposition on Conductive Hydrophobic Surfaces for Tuning and Quantitation in UV-MALDI Mass Spectrometry
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Journal Article
Liquid Matrix Deposition on Conductive Hydrophobic Surfaces for Tuning and Quantitation in UV-MALDI Mass Spectrometry
2007
Overview
With its highly fluctuating ion production matrix-assisted laser desorption/ionization (MALDI) poses many practical challenges for its application in mass spectrometry. Instrument tuning and quantitative ion abundance measurements using ion signal alone depend on a stable ion beam. Liquid MALDI matrices have been shown to be a promising alternative to the commonly used solid matrices. Their application in areas where a stable ion current is essential has been discussed but only limited data have been provided to demonstrate their practical use and advantages in the formation of stable MALDI ion beams. In this article we present experimental data showing high MALDI ion beam stability over more than two orders of magnitude at high analytical sensitivity (low femtomole amount prepared) for quantitative peptide abundance measurements and instrument tuning in a MALDI Q-TOF mass spectrometer. Samples were deposited on an inexpensive conductive hydrophobic surface and shrunk to droplets <10 nL in size. By using a sample droplet <10 nL it was possible to acquire data from a single irradiated spot for roughly 10,000 shots with little variation in ion signal intensity at a laser repetition rate of 5–20 Hz.
Publisher
Elsevier Inc,Elsevier Science,Springer Nature B.V
Subject
/ Aminoacids, peptides. Hormones. Neuropeptides
/ Analytical, structural and metabolic biochemistry
/ Biological and medical sciences
/ Exact sciences and technology
/ Fundamental and applied biological sciences. Psychology
/ Hydrophobic and Hydrophilic Interactions
/ Lasers
/ Peptide Fragments - chemistry
/ Proteins
/ Spectrometric and optical methods
/ Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - instrumentation
/ Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
/ Tuning
