Asset Details
Do you wish to reserve the book?
Cell type-dependent uptake, localization, and cytotoxicity of 1.9 nm gold nanoparticles
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Cell type-dependent uptake, localization, and cytotoxicity of 1.9 nm gold nanoparticles
Do you wish to request the book?
Cell type-dependent uptake, localization, and cytotoxicity of 1.9 nm gold nanoparticles
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Cell type-dependent uptake, localization, and cytotoxicity of 1.9 nm gold nanoparticles
2012
Overview
This follow-up study aims to determine the physical parameters which govern the differential radiosensitization capacity of two tumor cell lines and one immortalized normal cell line to 1.9 nm gold nanoparticles. In addition to comparing the uptake potential, localization, and cytotoxicity of 1.9 nm gold nanoparticles, the current study also draws on comparisons between nanoparticle size and total nanoparticle uptake based on previously published data.
We quantified gold nanoparticle uptake using atomic emission spectroscopy and imaged intracellular localization by transmission electron microscopy. Cell growth delay and clonogenic assays were used to determine cytotoxicity and radiosensitization potential, respectively. Mechanistic data were obtained by Western blot, flow cytometry, and assays for reactive oxygen species.
Gold nanoparticle uptake was preferentially observed in tumor cells, resulting in an increased expression of cleaved caspase proteins and an accumulation of cells in sub G(1) phase. Despite this, gold nanoparticle cytotoxicity remained low, with immortalized normal cells exhibiting an LD(50) concentration approximately 14 times higher than tumor cells. The surviving fraction for gold nanoparticle-treated cells at 3 Gy compared with that of untreated control cells indicated a strong dependence on cell type in respect to radiosensitization potential.
Gold nanoparticles were most avidly endocytosed and localized within cytoplasmic vesicles during the first 6 hours of exposure. The lack of significant cytotoxicity in the absence of radiation, and the generation of gold nanoparticle-induced reactive oxygen species provide a potential mechanism for previously reported radiosensitization at megavoltage energies.
Publisher
Taylor & Francis Ltd,Dove Press,Dove Medical Press
Subject
Apoptosis Regulatory Proteins - metabolism
/ Breast Neoplasms - drug therapy
/ Breast Neoplasms - metabolism
/ Cell Proliferation - drug effects
/ Cell Survival - drug effects
/ Gold
/ Humans
/ Male
/ Metal Nanoparticles - administration & dosage
/ Metal Nanoparticles - chemistry
/ Metal Nanoparticles - toxicity
/ Microscopy, Electron, Transmission
/ Prostatic Neoplasms - drug therapy
/ Prostatic Neoplasms - metabolism
