Asset Details
Do you wish to reserve the book?
Integrated Biomimetic 2D-LC and Permeapad® Assay for Profiling the Transdermal Diffusion of Pharmaceutical Compounds
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Integrated Biomimetic 2D-LC and Permeapad® Assay for Profiling the Transdermal Diffusion of Pharmaceutical Compounds
Do you wish to request the book?
Integrated Biomimetic 2D-LC and Permeapad® Assay for Profiling the Transdermal Diffusion of Pharmaceutical Compounds
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Integrated Biomimetic 2D-LC and Permeapad® Assay for Profiling the Transdermal Diffusion of Pharmaceutical Compounds
2026
Overview
A comprehensive two-dimensional liquid chromatography platform (LC × LC) was developed and validated for dermal permeability studies. In this implementation, the two separation dimensions were applied to mimic the layered structure of human skin: a ceramide-like stationary phase in the first dimension (1D) to simulate the lipid-rich epidermis, and an immobilized artificial membrane (IAM) phase in the second (2D) to emulate the dermis. Experimental conditions were optimised to reflect the microenvironment of the in vivo skin. For validation purposes, 43 pharmaceutical and cosmetic compounds whose transdermal permeability coefficients (log Kp) were known from the scientific literature were selected as model solutes. A good degree of separation was achieved across the whole dataset, and affinity profiles correlated with transdermal passage properties, suggesting that retention within specific chromatographic ranges may be predictive of skin permeation. To complement this approach, mass diffusion measurements were also conducted using Permeapad® 96-well plates and LC was performed on a narrow bore column in MS-friendly conditions. These log Kp values were compared against both in vivo and chromatographic retention data. The combined use of these techniques offers a strategic framework for profiling new chemical entities for their dermal absorption in a manner that is both ethically compliant and eco-sustainable.
Publisher
MDPI AG,Multidisciplinary Digital Publishing Institute (MDPI)
