Journal Article

Characterization of a protein of the plastid inner envelope having homology to animal inorganic phosphate, chloride and organic-anion transporters

Petétot, Jean MacDonald-Comber, Rochat-Hacker, Sylvie, Poirier, Yves, Roth, Christian, Menzel, Gerhard

2004

Overview

A protein from Arabidopsis thaliana (L.) Heynh. showing homology to animal proteins of the NaPi-1 family, involved in the transport of inorganic phosphate, chloride, glutamate and sialic acid, has been characterized. This protein, named ANTR2 (for anion transporters) was shown by chloroplast subfractionation to be localized to the plastid inner envelope in both A. thaliana and Spinacia oleracea (L.). Immunolocalization revealed that ANTR2 was expressed in the leaf mesophyll cells as well as in the developing embryo at the upturned-U stage. Five additional homologues of ANTR2 are found in the Arabidopsis genome, of which one was shown by green fluorescent protein (GFP) fusion to be also located in the chloroplast. All ANTR proteins share homology to the animal NaPi-1 family, as well as to other organic-anion transporters that are members of the Anion:Cation Symporter (ACS) family, and share the main features of transporters from this family, including the presence of 12 putative transmembrane domains and of a 7-amino acid motif in the fourth putative transmembrane domain. ANTR2 thus represent a novel protein of the plastid inner envelope that is likely to be involved in anion transport.

• Publisher: Springer-Verlag,Springer,Springer Nature B.V
• Subject: Absorption. Translocation of ions and substances. Permeability / Agronomy. Soil science and plant productions / Amino Acid Sequence / amino acid sequences / Amino acids / Animals / Anion Transport Proteins / Anion Transport Proteins - chemistry / Anion Transport Proteins - genetics / Anions / Antibodies / Arabidopsis / Arabidopsis - genetics / Arabidopsis - metabolism / Arabidopsis Proteins / Arabidopsis Proteins - genetics / Arabidopsis Proteins - metabolism / Arabidopsis thaliana / Biological and medical sciences / Caenorhabditis elegans / Caenorhabditis elegans - genetics / Carrier Proteins / Carrier Proteins - chemistry / Carrier Proteins - genetics / Cell membranes / Cell physiology / chemistry / Chlorides / Chloroplasts / Cloning, Molecular / Complementary DNA / Economic plant physiology / Escherichia coli / Escherichia coli - genetics / Escherichia coli - metabolism / Fundamental and applied biological sciences. Psychology / gene expression regulation / genetics / Humans / K Cl- Cotransporters / Membrane Transport Proteins / metabolism / Molecular Sequence Data / Nutrition. Photosynthesis. Respiration. Metabolism / Phosphate Transport Proteins / Phosphate Transport Proteins - chemistry / Phosphate Transport Proteins - genetics / Phosphates / Plant physiology and development / plant proteins / Plasma membrane and permeation / Plastids / Plastids - genetics / Proteins / Sequence Alignment / Sequence Homology, Amino Acid / spinach / Spinacia oleracea / symporters / Symporters - chemistry / Symporters - genetics / vegetable crops / Vesicular Glutamate Transport Protein 1 / Vesicular Transport Proteins