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Rapid lateral flow immunoassay for the fluorescence detection of SARS-CoV-2 RNA
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Rapid lateral flow immunoassay for the fluorescence detection of SARS-CoV-2 RNA
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Journal Article
Rapid lateral flow immunoassay for the fluorescence detection of SARS-CoV-2 RNA
2020
Overview
The coronavirus disease 2019 (COVID-19) pandemic has highlighted the need for rapid and accurate nucleic acid detection at the point of care. Here, we report an amplification-free nucleic acid immunoassay, implemented on a lateral flow strip, for the fluorescence detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in less than one hour. The assay uses DNA probes that are designed to bind to the conserved open reading frame 1ab (ORF1ab), envelope protein (E) and the nucleocapsid (N) regions of the SARS-CoV-2 genome, and a fluorescent-nanoparticle-labelled monoclonal antibody that binds to double-stranded DNA–RNA hybrids. In a multi-hospital randomized double-blind trial involving 734 samples (593 throat swabs and 141 sputum) provided by 670 individuals, the assay achieved sensitivities of 100% and specificities of 99% for both types of sample (ground truth was determined using quantitative PCR with reverse transcription). The inexpensive amplification-free detection of SARS-CoV-2 RNA should facilitate the rapid diagnosis of COVID-19 at the point of care.
A nucleic acid immunoassay implemented on a lateral flow strip accurately detects SARS-CoV-2 RNA in less than one hour via a fluorescence readout.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ 9/10
/ Antibodies, Monoclonal - immunology
/ Biomedical and Life Sciences
/ Biomedical Engineering/Biotechnology
/ COVID-19
/ DNA
/ Genomes
/ Humans
/ Hybrids
/ Molecular Diagnostic Techniques - methods
/ Nucleic Acid Amplification Techniques - methods
/ Pandemics - prevention & control
/ RNA
/ Severe acute respiratory syndrome coronavirus 2
/ Sputum
/ Strip
