Asset Details
Do you wish to reserve the book?
Investigation of the Properties of Non-gypsy Suppressor of Hairy-wing-Binding Sites
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Investigation of the Properties of Non-gypsy Suppressor of Hairy-wing-Binding Sites
Do you wish to request the book?
Investigation of the Properties of Non-gypsy Suppressor of Hairy-wing-Binding Sites
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Investigation of the Properties of Non-gypsy Suppressor of Hairy-wing-Binding Sites
2008
Overview
Insulators define interactions between transcriptional control elements in eukaryotic genomes. The gypsy insulator found in the gypsy retrovirus binds the zinc-finger Suppressor of Hairy-wing [Su(Hw)] protein that associates with hundreds of non-gypsy regions throughout the Drosophila genome. Models of insulator function predict that the gypsy insulator forms chromatin loop domains through interactions with endogenous Su(Hw) insulators (SIs) to limit the action of transcriptional control elements. Here we study SI 62D and show that interactions occur between two SI 62D elements, but not between SI 62D and the gypsy insulator, limiting the scope of genomic gypsy insulator interactions. Enhancer blocking by SI 62D requires fewer Su(Hw)-binding sites than needed for gypsy insulator function, with these target regions having distinct zinc-finger requirements for in vivo Su(Hw) association. These observations led to an investigation of the role of the Su(Hw) zinc-finger domain in insulator function. Using a combination of in vitro and in vivo studies, we find that this domain makes sequence-dependent and -independent contributions to in vivo chromosome association, but is not essential for enhancer or silencer blocking. These studies extend our understanding of the properties of Su(Hw) and the endogenous genomic regions to which this protein localizes.
Publisher
Genetics Soc America,Genetics Society of America
Subject
/ Animals
/ Bacterial Proteins - metabolism
/ Chromatin Immunoprecipitation
/ DNA
/ DNA-Binding Proteins - metabolism
/ Drosophila melanogaster - genetics
/ Drosophila Proteins - metabolism
/ Enhancer Elements, Genetic - genetics
/ Eye
/ Genetics
/ Insulator Elements - genetics
/ protein-protein interactions
/ Proteins
/ Repressor Proteins - metabolism
/ Serine Endopeptidases - metabolism
/ Silencer Elements, Transcriptional
/ Silencer Elements, Transcriptional - genetics
/ transcriptional control elements
/ Zinc
