Asset Details
Do you wish to reserve the book?
Changes in Microbiota Profiles After Prolonged Frozen Storage of Stool Suspensions
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Changes in Microbiota Profiles After Prolonged Frozen Storage of Stool Suspensions
Do you wish to request the book?
Changes in Microbiota Profiles After Prolonged Frozen Storage of Stool Suspensions
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Changes in Microbiota Profiles After Prolonged Frozen Storage of Stool Suspensions
2020
Overview
Fecal microbiota transplantation (FMT) is recommended as safe and effective treatment for recurrent
infections. Freezing the FMT preparation simplifies the process, allowing a single stool sample to be used for multiple receivers and over an extended period of time. We aimed to assess the effect of long-term frozen storage on bacterial taxonomic profiles of a stool suspension prepared for FMT.
DNA was extracted from a stool suspension before freezing and sequentially during the 18-month storage period at -80°C. Two different protocols were used for DNA extraction. The first relied on a classical mechanical and chemical cell disruption to extract both intra- and extracellular DNA; the second included specific pre-treatments aimed at removing free DNA and DNA from human and damaged bacterial cells. Taxonomic profiling of bacterial communities was performed by sequencing of V3-V4 16S rRNA gene amplicons.
Microbiota profiles obtained by whole DNA extraction procedure remained relatively stable during frozen storage. When DNA extraction procedure included specific pre-treatments, microbiota similarity between fresh and frozen samples progressively decreased with longer frozen storage times; notably, the abundance of Bacteroidetes decreased in a storage duration-dependent manner. The abundance of Firmicutes, the main butyrate producers in the colon, were not much affected by frozen storage for up to 1 year.
Our data show that metataxonomic analysis of frozen stool suspensions subjected to specific pre-treatments prior to DNA extractions might provide an interesting indication of bacterial resistance to stress conditions and thus of chances of survival in FMT recipients.
Publisher
Frontiers Media SA,Frontiers Media S.A
Subject
/ Bacteria - isolation & purification
/ Bacteroidetes - isolation & purification
/ Cellular and Infection Microbiology
/ DNA
/ E coli
/ Fecal Microbiota Transplantation
/ Feces
/ Firmicutes - isolation & purification
/ Freezing
/ Genes
/ Genomics
/ Humans
/ RNA, Ribosomal, 16S - genetics
/ rRNA 16S
/ Software
/ stool
/ Taxonomy
