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Establishing functional giant Dictyostelium cells reveals front–rear polarity in intracellular signaling
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Establishing functional giant Dictyostelium cells reveals front–rear polarity in intracellular signaling
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Journal Article
Establishing functional giant Dictyostelium cells reveals front–rear polarity in intracellular signaling
2026
Overview
Intracellular signaling dynamics are often obscured by the spatial and temporal limitations of cell size. Here, we developed a method to enlarge
Dictyostelium discoideum
cells by partial cytokinesis inhibition, generating multinucleated yet functional giant cells. These cells retained chemotactic signaling, polarity, and motility, enabling high-resolution live-cell imaging. Using fluorescent probes for cAMP and Ca
2+
, we uncovered a directional, front-to-rear propagation of cAMP signaling and a biphasic Ca
2+
response coordinated with actin wave dynamics. Grid-based mapping revealed asymmetric cAMP synthesis and decay kinetics, and vesicle localization suggested spatially regulated cAMP secretion. Our findings demonstrate that intracellular signaling involves self-organized, spatially structured propagation events aligned with cellular polarity. The giant cell platform offers a powerful and generalizable strategy for dissecting the spatiotemporal logic of single-cell signaling.
A method to enlarge living
Dictyostelium
cells by selectively inhibiting cytokinesis while preserving cellular functions enables direct visualization of directional cAMP and Ca
2+
signal propagation in single cells.
