Journal Article

The protective effect of icariin and phosphorylated icariin against LPS-induced intestinal goblet cell dysfunction

Wang, Jian, Ma, Haoyue, Xu, Yuwei, Jin, Meilan, Xiong, Wen, Zhang, Zhu, Wang, Zili

2020

Overview

In this study, we used LS174T cells as a model to investigate the protective effects of icariin and phosphorylated icariin on LPS-induced goblet cell dysfunction. Our results indicated that icariin and phosphorylated icariin increased the cell viability and decreased lactate dehydrogenase activity in LPS-treated LS174T cells. Icariin and phosphorylated icariin attenuated LPS-induced changes in mucin 2 synthesis and secretion. Besides, Icariin and phosphorylated icariin reduced the levels of ROS, MDA, and H2O2 and increased the activity of SOD, GPx, CAT, and T-AOC in LPS-treated LS174T cells. Moreover, the levels of IL-1β, IL-6, IL-8, and TNF-α were reduced in the Icariin and phosphorylated icariin group. Furthermore, Icariin and phosphorylated icariin decreased gene abundance or enzyme activity of Bip, XBP1, GRP78, CHOP, caspase-3, and caspase-4 in LPS-treated LS174T cells. Our data suggest that Icariin and phosphorylated icariin effectively attenuate LPS-induced intestinal goblet cell function damage through regulating oxidative stress, inflammation, apoptosis, and mucin expression.

• Publisher: SAGE Publications,SAGE PUBLICATIONS, INC,SAGE Publishing
• Subject: Apoptosis / Apoptosis - drug effects / Apoptosis - genetics / Caspase 3 - genetics / Caspase 3 - metabolism / Caspase-3 / Caspase-4 / Caspases, Initiator - genetics / Caspases, Initiator - metabolism / Catalase - metabolism / Cell Proliferation - drug effects / Cell Survival - drug effects / Cell viability / Enzymatic activity / Flavonoids - metabolism / Flavonoids - pharmacology / Glutathione Peroxidase - metabolism / Goblet Cells - drug effects / Goblet Cells - enzymology / Goblet Cells - metabolism / Heat-Shock Proteins - genetics / Heat-Shock Proteins - metabolism / Humans / Hydrogen peroxide / Hydrogen Peroxide - metabolism / IL-1β / Inflammation - genetics / Inflammation - metabolism / Interleukin 6 / Interleukin 8 / Interleukin-1beta - metabolism / Interleukin-6 - metabolism / Interleukin-8 - metabolism / Intestine / L-Lactate dehydrogenase / L-Lactate Dehydrogenase - metabolism / Lactic acid / Lipopolysaccharides / Lipopolysaccharides - pharmacology / Malondialdehyde - metabolism / Mucin / Mucin-2 - biosynthesis / Mucin-2 - metabolism / Original / Oxidative stress / Phosphorylation / Reactive Oxygen Species - metabolism / Superoxide Dismutase-1 - metabolism / Transcription Factor CHOP - genetics / Transcription Factor CHOP - metabolism / Tumor Necrosis Factor-alpha - metabolism / Tumor necrosis factor-α / X-Box Binding Protein 1 - genetics / X-Box Binding Protein 1 - metabolism