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Micropipette aspiration as a tool for single‐particle X‐ray imaging and diffraction
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Micropipette aspiration as a tool for single‐particle X‐ray imaging and diffraction
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Journal Article
Micropipette aspiration as a tool for single‐particle X‐ray imaging and diffraction
2023
Overview
A sample environment and manipulation tool is presented for single‐particle X‐ray experiments in an aqueous environment. The system is based on a single water droplet, positioned on a substrate that is structured by a hydrophobic and hydrophilic pattern to stabilize the droplet position. The substrate can support several droplets at a time. Evaporation is prevented by covering the droplet by a thin film of mineral oil. In this windowless fluid which minimizes background signal, single particles can be probed and manipulated by micropipettes, which can easily be inserted and steered in the droplet. Holographic X‐ray imaging is shown to be well suited to observe and monitor the pipettes, as well as the droplet surface and the particles. Aspiration and force generation are also enabled based on an application of controlled pressure differences. Experimental challenges are addressed and first results are presented, obtained at two different undulator endstations with nano‐focused beams. Finally, the sample environment is discussed in view of future coherent imaging and diffraction experiments with synchrotron radiation and single X‐ray free‐electron laser pulses. Micropipette aspiration has been combined with a windowless hydrated sample environment for single‐particle X‐ray analysis. A water droplet serves as a `chamber', covered by a mineral oil to prevent evaporation. In the droplet, particles can be aspirated and manipulated by one or two micropipettes. The system was evaluated on two undulator beamlines, using grains, giant lipid vesicles, colloids and macrophages as test objects. Holographic near‐field images were recorded.
Publisher
International Union of Crystallography,John Wiley & Sons, Inc
Subject
