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An overview of ELISA: a review and update on best laboratory practices for quantifying peptides and proteins in biological fluids
by
Emre, Elif
, Aydin, Suleyman
, Aydin, Mustafa Ata
, Sahin, İbrahim
, Ugur, Kader
, Akbulut, Taner
, Cinar, Vedat
in
Body Fluids - chemistry
/ Body Fluids - metabolism
/ Enzyme-Linked Immunosorbent Assay - methods
/ Enzyme-Linked Immunosorbent Assay - standards
/ Humans
/ Narrative Review
/ Peptides - analysis
/ Proteins - analysis
2025
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An overview of ELISA: a review and update on best laboratory practices for quantifying peptides and proteins in biological fluids
by
Emre, Elif
, Aydin, Suleyman
, Aydin, Mustafa Ata
, Sahin, İbrahim
, Ugur, Kader
, Akbulut, Taner
, Cinar, Vedat
in
Body Fluids - chemistry
/ Body Fluids - metabolism
/ Enzyme-Linked Immunosorbent Assay - methods
/ Enzyme-Linked Immunosorbent Assay - standards
/ Humans
/ Narrative Review
/ Peptides - analysis
/ Proteins - analysis
2025
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Do you wish to request the book?
An overview of ELISA: a review and update on best laboratory practices for quantifying peptides and proteins in biological fluids
by
Emre, Elif
, Aydin, Suleyman
, Aydin, Mustafa Ata
, Sahin, İbrahim
, Ugur, Kader
, Akbulut, Taner
, Cinar, Vedat
in
Body Fluids - chemistry
/ Body Fluids - metabolism
/ Enzyme-Linked Immunosorbent Assay - methods
/ Enzyme-Linked Immunosorbent Assay - standards
/ Humans
/ Narrative Review
/ Peptides - analysis
/ Proteins - analysis
2025
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An overview of ELISA: a review and update on best laboratory practices for quantifying peptides and proteins in biological fluids
Journal Article
An overview of ELISA: a review and update on best laboratory practices for quantifying peptides and proteins in biological fluids
2025
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Overview
The enzyme-linked immunosorbent assay (ELISA) detects antigen-antibody interactions by using enzyme-labelled conjugates and enzyme substrates that generate colour changes. This review aims to provide an overview of ELISA, its various types, and its applications in detecting metabolites in biological fluids. The article discusses the history of the assay, its underlying principles and procedures, common ELISA protocols, and the most accurate and reliable techniques for measuring peptide molecules in biological fluids. Additionally, we emphasize best laboratory practices to achieve consistent, high-quality results and outline the essential materials for setting up an ELISA laboratory, drawing from our over 30 years of experience in the field.
Publisher
SAGE Publications,Sage Publications Ltd,SAGE Publishing
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