Journal Article

The genome of opportunistic fungal pathogen Fusarium oxysporum carries a unique set of lineage-specific chromosomes

Guo, Li, Zeng, Qiandong, Di Pietro, Antonio, McAdam, Alexander J., Shea, Terrance, Schwartz, David C., DeIulio, Gregory A., Yang, He, Rep, Martijn, Turra, David, Wiederhold, Nathan, Kistler, H. Corby, Coleman, Jeffrey J., Youngster, Ilan, Pearlman, Eric, Young, Sarah, Zhou, Shiguo, Ma, Li-Jun, Broz, Karen, Donnell, Kerry O, Ayhan, Dilay Hazal, Savinov, Sergey, Zhang, Yong

2020

Overview

Fusarium oxysporum is a cross-kingdom fungal pathogen that infects plants and humans. Horizontally transferred lineage-specific (LS) chromosomes were reported to determine host-specific pathogenicity among phytopathogenic F. oxysporum . However, the existence and functional importance of LS chromosomes among human pathogenic isolates are unknown. Here we report four unique LS chromosomes in a human pathogenic strain NRRL 32931, isolated from a leukemia patient. These LS chromosomes were devoid of housekeeping genes, but were significantly enriched in genes encoding metal ion transporters and cation transporters. Homologs of NRRL 32931 LS genes, including a homolog of ceruloplasmin and the genes that contribute to the expansion of the alkaline pH-responsive transcription factor PacC/Rim1p, were also present in the genome of NRRL 47514, a strain associated with Fusarium keratitis outbreak. This study provides the first evidence, to our knowledge, for genomic compartmentalization in two human pathogenic fungal genomes and suggests an important role of LS chromosomes in niche adaptation. Zhang, Yang et al. compare a Fusarium oxysporum isolate obtained clinically to a phytopathogenic strain to examine transfer of lineage-specific chromosomes in determining host specificity. They find four unique lineage-specific chromosomes that seem to contribute to fungal adaptation to human hosts.

• Publisher: Nature Publishing Group UK,Nature Publishing Group
• Subject: 38 / 38/39 / 45 / 45/23 / 45/77 / 45/91 / 631/114/2785 / 631/326/193/2541 / 631/326/421 / Amino Acid Sequence / Biology / Biomedical and Life Sciences / Ceruloplasmin / Chromosomes / Chromosomes, Fungal / Fungal Proteins - chemistry / Fungal Proteins - genetics / Fusariosis - microbiology / Fusarium - genetics / Fusarium - isolation & purification / Fusarium oxysporum / Gene Expression Regulation, Fungal / Genome, Fungal / Genomes / Host specificity / Humans / Keratitis / Life Sciences / Metal ions / Models, Molecular / Opportunist infection / Opportunistic Infections - microbiology / Pathogenicity / Pathogens / Phylogeny / Protein Conformation / Structure-Activity Relationship