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Molecular resolution imaging by repetitive optical selective exposure
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Journal Article
Molecular resolution imaging by repetitive optical selective exposure
2019
Overview
We introduce an interferometric single-molecule localization method for super-resolution fluorescence microscopy. Fluorescence molecules are located by the intensities of multiple excitation patterns of an interference fringe, providing around a twofold improvement in the localization precision compared with the conventional imaging with the same photon budget. We demonstrate this technique by resolving nanostructures down to 5 nm in size over a large 25 × 25 μm2 field of view.
Publisher
Nature Publishing Group
Subject
