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A large genome center's improvements to the Illumina sequencing system
by
Scally, Aylwyn
, Smith, Frances
, Quail, Michael A
, Kozarewa, Iwanka
, Swerdlow, Harold
, Stephens, Philip J
, Durbin, Richard
, Turner, Daniel J
in
Academies and Institutes
/ Bioinformatics
/ Biological Microscopy
/ Biological Techniques
/ Biomedical and Life Sciences
/ Biomedical Engineering/Biotechnology
/ Chromosome Mapping - instrumentation
/ Data analysis
/ DNA sequencers
/ Equipment Design
/ Genomic libraries
/ Genomics
/ Genomics - instrumentation
/ Life Sciences
/ Management
/ Parallel processing
/ perspective
/ Polymerase chain reaction
/ Polymerase Chain Reaction - instrumentation
/ Proteomics
/ Research centers
/ Research methodology
/ Sequence Analysis, DNA - instrumentation
2008
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A large genome center's improvements to the Illumina sequencing system
by
Scally, Aylwyn
, Smith, Frances
, Quail, Michael A
, Kozarewa, Iwanka
, Swerdlow, Harold
, Stephens, Philip J
, Durbin, Richard
, Turner, Daniel J
in
Academies and Institutes
/ Bioinformatics
/ Biological Microscopy
/ Biological Techniques
/ Biomedical and Life Sciences
/ Biomedical Engineering/Biotechnology
/ Chromosome Mapping - instrumentation
/ Data analysis
/ DNA sequencers
/ Equipment Design
/ Genomic libraries
/ Genomics
/ Genomics - instrumentation
/ Life Sciences
/ Management
/ Parallel processing
/ perspective
/ Polymerase chain reaction
/ Polymerase Chain Reaction - instrumentation
/ Proteomics
/ Research centers
/ Research methodology
/ Sequence Analysis, DNA - instrumentation
2008
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A large genome center's improvements to the Illumina sequencing system
by
Scally, Aylwyn
, Smith, Frances
, Quail, Michael A
, Kozarewa, Iwanka
, Swerdlow, Harold
, Stephens, Philip J
, Durbin, Richard
, Turner, Daniel J
in
Academies and Institutes
/ Bioinformatics
/ Biological Microscopy
/ Biological Techniques
/ Biomedical and Life Sciences
/ Biomedical Engineering/Biotechnology
/ Chromosome Mapping - instrumentation
/ Data analysis
/ DNA sequencers
/ Equipment Design
/ Genomic libraries
/ Genomics
/ Genomics - instrumentation
/ Life Sciences
/ Management
/ Parallel processing
/ perspective
/ Polymerase chain reaction
/ Polymerase Chain Reaction - instrumentation
/ Proteomics
/ Research centers
/ Research methodology
/ Sequence Analysis, DNA - instrumentation
2008
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A large genome center's improvements to the Illumina sequencing system
Journal Article
A large genome center's improvements to the Illumina sequencing system
2008
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Overview
The Wellcome Trust Sanger Institute is one of the world's largest genome centers, and a substantial amount of our sequencing is performed with 'next-generation' massively parallel sequencing technologies: in June 2008 the quantity of purity-filtered sequence data generated by our Genome Analyzer (Illumina) platforms reached 1 terabase, and our average weekly Illumina production output is currently 64 gigabases. Here we describe a set of improvements we have made to the standard Illumina protocols to make the library preparation more reliable in a high-throughput environment, to reduce bias, tighten insert size distribution and reliably obtain high yields of data.
Publisher
Nature Publishing Group US,Nature Publishing Group
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