Journal Article

Integrating coaxial electrospinning and 3D printing technologies for the development of biphasic porous scaffolds enabling spatiotemporal control in tumor ablation and osteochondral regeneration

Wu, Jing, Zhou, Haichao, Xu, Yong, Yang, Yunfeng, Li, Junjun, Zhao, Shitong, Li, Chunlin, He, Wenbao, Li, Zhendong, Xia, Huitang

2024

Overview

The osteochondral defects (OCDs) resulting from the treatment of giant cell tumors of bone (GCTB) often present two challenges for clinicians: tumor residue leading to local recurrence and non-healing of OCDs. Therefore, this study focuses on developing a double-layer PGPC-PGPH scaffold using shell-core structure nanofibers to achieve “spatiotemporal control” for treating OCDs caused by GCTB. It addresses two key challenges: eliminating tumor residue after local excision and stimulating osteochondral regeneration in non-healing OCD cases. With a shell layer of protoporphyrin IX (PpIX)/gelatin (GT) and inner cores containing chondroitin sulfate (CS)/poly(lactic-co-glycolic acid) (PLGA) or hydroxyapatite (HA)/PLGA, coaxial electrospinning technology was used to create shell-core structured PpIX/GT-CS/PLGA and PpIX/GT-HA/PLGA nanofibers. These nanofibers were shattered into nano-scaled short fibers, and then combined with polyethylene oxide and hyaluronan to formulate distinct 3D printing inks. The upper layer consists of PpIX/GT-CS/PLGA ink, and the lower layer is made from PpIX/GT-HA/PLGA ink, allowing for the creation of a double-layer PGPC-PGPH scaffold using 3D printing technique. After GCTB lesion removal, the PGPC-PGPH scaffold is surgically implanted into the OCDs. The sonosensitizer PpIX in the shell layer undergoes sonodynamic therapy to selectively damage GCTB tissue, effectively eradicating residual tumors. Subsequently, the thermal effect of sonodynamic therapy accelerates the shell degradation and release of CS and HA within the core layer, promoting stem cell differentiation into cartilage and bone tissues at the OCD site in the correct anatomical position. This innovative scaffold provides temporal control for anti-tumor treatment followed by tissue repair and spatial control for precise osteochondral regeneration. [Display omitted] •Utilizing coaxial electrospinning, we generated shell-core structured nanofibers comprising a PpIX/GT shell layer and inner cores containing CS/PLGA or HA/PLGA.•By converting these nanofibers into short fibers, we developed distinct 3D printing inks, facilitating the creation of a double-layer PGPC-PGPH scaffold via advanced 3D printing methods.•The sonosensitizer PpIX embedded in the shell layer demonstrates targeted sonodynamic therapy, effectively damaging GCTB cells.•The specific inducing factors within the inner core precisely direct chondrogenesis in the PGPC layer and osteogenesis in the PGPH layer.•The PGPC-PGPH scaffold offers temporal control by eradicating GCTB cells followed by facilitating osteochondral regeneration, while also ensuring precise regeneration of upper cartilage and underlying bone through spatial control.

• Publisher: Elsevier B.V,KeAi Publishing,KeAi Communications Co., Ltd
• Subject: Double-layered scaffold / Giant cell tumors of bone / Osteochondral regeneration / Shell-core structure / Spatiotemporal control