Asset Details
Do you wish to reserve the book?
Real-time selective sequencing using nanopore technology
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Real-time selective sequencing using nanopore technology
Do you wish to request the book?
Real-time selective sequencing using nanopore technology
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Real-time selective sequencing using nanopore technology
2016
Overview
Read Until allows real-time selective sequencing on a nanopore sequencer, enabling applications such as target enrichment and amplicon normalization.
The Oxford Nanopore Technologies MinION sequencer enables the selection of specific DNA molecules for sequencing by reversing the driving voltage across individual nanopores. To directly select molecules for sequencing, we used dynamic time warping to match reads to reference sequences. We demonstrate our open-source Read Until software in real-time selective sequencing of regions within small genomes, individual amplicon enrichment and normalization of an amplicon set.
Publisher
Nature Publishing Group US,Nature Publishing Group
Subject
/ Bacteriophage lambda - genetics
/ Biomedical and Life Sciences
/ Biomedical Engineering/Biotechnology
/ DNA
/ Genomes
/ Genomics
/ High-Throughput Nucleotide Sequencing - methods
/ Humans
/ Methods
/ Nucleic Acid Amplification Techniques - methods
/ Repetitive Sequences, Nucleic Acid
