Asset Details
Do you wish to reserve the book?
Click chemistry–enabled CRISPR screening reveals GSK3 as a regulator of PLD signaling
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Click chemistry–enabled CRISPR screening reveals GSK3 as a regulator of PLD signaling
Do you wish to request the book?
Click chemistry–enabled CRISPR screening reveals GSK3 as a regulator of PLD signaling
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Click chemistry–enabled CRISPR screening reveals GSK3 as a regulator of PLD signaling
2021
Overview
Enzymes that produce second messengers are highly regulated. Revealing the mechanisms underlying such regulation is critical to understanding both how cells achieve specific signaling outcomes and return to homeostasis following a particular stimulus. Pooled genome-wide CRISPR screens are powerful unbiased approaches to elucidate regulatory networks, their principal limitation being the choice of phenotype selection. Here, we merge advances in bioorthogonal fluorescent labeling and CRISPR screening technologies to discover regulators of phospholipase D (PLD) signaling, which generates the potent lipid second messenger phosphatidic acid. Our results reveal glycogen synthase kinase 3 as a positive regulator of protein kinase C and PLD signaling. More generally, this work demonstrates how bioorthogonal, activity-based fluorescent tagging can expand the power of CRISPR screening to uncover mechanisms regulating specific enzyme-driven signaling pathways in mammalian cells.
Publisher
National Academy of Sciences
Subject
/ Clustered Regularly Interspaced Short Palindromic Repeats
/ CRISPR
/ CRISPR-Cas Systems - genetics
/ Genomes
/ Glycogen
/ Glycogen Synthase Kinase 3 - metabolism
/ Glycogen Synthase Kinase 3 - physiology
/ Humans
/ Kinases
/ Lipids
/ Phosphatidic Acids - metabolism
/ Phospholipase D - metabolism
/ Phospholipase D - physiology
/ Protein Kinase C-alpha - metabolism
