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Methods for Photocrosslinking Alginate Hydrogel Scaffolds with High Cell Viability
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Methods for Photocrosslinking Alginate Hydrogel Scaffolds with High Cell Viability
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Journal Article
Methods for Photocrosslinking Alginate Hydrogel Scaffolds with High Cell Viability
2011
Overview
Methods for seeding high-viability (>85%) three-dimensional (3D) alginate–chondrocyte hydrogel scaffolds are presented that employ photocrosslinking of methacrylate-modified alginate with the photoinitiator VA-086. Comparison with results from several other photoinitiators, including Irgacure 2959, highlights the role of solvent, ultraviolet exposure, and photoinitiator cytotoxicity on process viability of bovine chondrocytes in two-dimensional culture. The radicals generated from VA-086 photodissociation are shown to be noncytotoxic at w/v concentrations up to 1.5%, enabling photocrosslinking without significant cell death. The applicability of these photoinitiators for generating 3D tissue-engineered constructs is evaluated by measuring cell viability in 3D constructs with aggregate moduli in the 10–20 kPa range. Hydrogels with encapsulated bovine chondrocytes were constructed with >85% viability using VA-086. While the commonly used Irgacure 2959 is noncytotoxic in its native state and crosslinks the alginate at weight fractions much lower than VA-086, the cytotoxicity of IRG2959's photogenerated radical leads to viabilities below 70% in the conditions tested.
Publisher
Mary Ann Liebert, Inc,SAGE Publications
Subject
/ Animals
/ Azo Compounds - pharmacology
/ Cartilage, Articular - cytology
/ Cattle
/ Cell Aggregation - drug effects
/ Cell Aggregation - radiation effects
/ Cell Survival - drug effects
/ Cell Survival - radiation effects
/ Chondrocytes - radiation effects
/ Cross-Linking Reagents - pharmacology
/ Glucuronic Acid - pharmacology
/ Hexuronic Acids - pharmacology
/ Hydrogel, Polyethylene Glycol Dimethacrylate - pharmacology
/ Light
